ArticleThe Biochemical journal2001
Regulation of Ca2+-release-activated Ca2+ current (Icrac) by ryanodine receptors in inositol 1,4,5-trisphosphate-receptor-deficient DT40 cells.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 60 citations in OpenAlex.
- Roles of NADMolecules (Basel, Switzerland) · 2020Review
- Structural Mechanisms of Store-Operated and Mitochondrial Calcium Regulation: Initiation Points for Drug Discovery.International journal of molecular sciences · 2020Review
- Does Cyclic ADP-Ribose (cADPR) Activate the Non-selective Cation Channel TRPM2?Frontiers in immunology · 2020Review
- Beyond the CRAC: Diversification of ion signaling in B cells.Immunological reviews · 2019Review
- L-type Ca2+ channel sparklets revealed by TIRF microscopy in mouse urinary bladder smooth muscle.PloS one · 2014Article
- Quantifying SOCE fluorescence measurements in mammalian muscle fibres. The effects of ryanodine and osmotic shocks.Journal of muscle research and cell motility · 2013Article
- Expression of Orai genes and I(CRAC) activation in the human retinal pigment epithelium.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2011Article
- Activation of store-operated I(CRAC) by hydrogen peroxide.Cell calcium · 2010Article
- 8-Bromo-cyclic inosine diphosphoribose: towards a selective cyclic ADP-ribose agonist.The Biochemical journal · 2009Article
- Mefloquine-induced disruption of calcium homeostasis in mammalian cells is similar to that induced by ionomycin.Antimicrobial agents and chemotherapy · 2008Article
- Role of InsP3 and ryanodine receptors in the activation of capacitative Ca2+ entry by store depletion or hypoxia in canine pulmonary arterial smooth muscle cells.British journal of pharmacology · 2007Article
- Coupling Ca2+ store release to Icrac channel activation in B lymphocytes requires the activity of Lyn and Syk kinases.The Journal of cell biology · 2007Article
- On the activation mechanism of store-operated calcium channels.Pflugers Archiv : European journal of physiology · 2006Review
- Extracellular NAD+ regulates intracellular calcium levels and induces activation of human granulocytes.The Biochemical journal · 2006Article
- Calcium signaling, ion channels and more. The DT40 system as a model of vertebrate ion homeostasis and cell physiology.Sub-cellular biochemistry · 2006Review
- Targeted expression of the inositol 1,4,5-triphosphate receptor (IP3R) ligand-binding domain releases Ca2+ via endogenous IP3R channels.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- TRPC3: a versatile transducer molecule that serves integration and diversification of cellular signals.Naunyn-Schmiedeberg's archives of pharmacology · 2005Review
- Activation of ryanodine receptors induces calcium influx in a neuroblastoma cell line lacking calcium influx factor activity.The Biochemical journal · 2005Article
- CCN3 and calcium signaling.Cell communication and signaling : CCS · 2003Article
- Ca2+ signalling and membrane current activated by cADPr in starfish oocytes.Pflugers Archiv : European journal of physiology · 2003Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Persistence of capacitative Ca(2+) influx in inositol 1,4,5-trisphosphate (IP(3)) receptor (IP(3)R)-deficient DT40 cells (DT40(IP(3)R-/-)) raises the question of whether gating of Ca(2+)-release activated Ca(2+) current (I(crac)) by conformational coupling to Ca(2+)-release channels is a general mechanism of gating of these channels. In the present work we examined the properties and mechanism of activation of I(crac) Ca(2+) current in wild-type and DT40(IP(3)R-/-) cells. In both cell types passive depletion of internal Ca(2+) stores by infusion of EGTA activated a Ca(2+) current with similar characteristics and time course. The current was highly Ca(2+)-selective and showed strong inward rectification, all typical of I(crac). The activator of ryanodine receptor (RyR), cADP-ribose (cADPR), facilitated activation of I(crac), and the inhibitors of the RyRs, 8-N-cADPR, ryanodine and Ruthenium Red, all inhibited I(crac) activation in DT40(IP(3)R-/-) cells, even after complete depletion of intracellular Ca(2+) stores by ionomycin. Wild-type and DT40(IP(3)R-/-) cells express RyR isoforms 1 and 3. RyR levels were adapted in DT40(IP(3)R-/-) cells to a lower RyR3/RyR1 ratio than in wild-type cells. These results suggest that IP(3)Rs and RyRs can efficiently gate I(crac) in DT40 cells and explain the persistence of I(crac) gating by internal stores in the absence of IP(3)Rs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.