ArticleMolecular biology of the cell2001
In vivo role for actin-regulating kinases in endocytosis and yeast epsin phosphorylation.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 73 citations in OpenAlex.
- Protein Phosphorylation Orchestrates Acclimations of Arabidopsis Plants to Environmental pH.Molecular & cellular proteomics : MCP · 2024Article
- Current thoughts on cellular functions of numb-associated kinases.Molecular biology reports · 2023Review
- Eps15/Pan1p is a master regulator of the late stages of the endocytic pathway.The Journal of cell biology · 2022Article
- Cooperative regulation of endocytic vesicle transport by yeast Eps15-like protein Pan1p and epsins.The Journal of biological chemistry · 2021Article
- Candida albicansmSphere · 2021Article
- Conserved Ark1-related kinases function in a TORC2 signaling network.Molecular biology of the cell · 2020Article
- Target of rapamycin complex 2-dependent phosphorylation of the coat protein Pan1 by Akl1 controls endocytosis dynamics inThe Journal of biological chemistry · 2018Article
- MoCAP proteins regulated by MoArk1-mediated phosphorylation coordinate endocytosis and actin dynamics to govern development and virulence of Magnaporthe oryzae.PLoS genetics · 2017Article
- TOR Complex 2-Regulated Protein Kinase Fpk1 Stimulates Endocytosis via Inhibition of Ark1/Prk1-Related Protein Kinase Akl1 in Saccharomyces cerevisiae.Molecular and cellular biology · 2017Article
- Article
- Actin and endocytosis in budding yeast.Genetics · 2015Review
- Αvβ3-integrin-mediated adhesion is regulated through an AAK1L- and EHD3-dependent rapid-recycling pathway.Journal of cell science · 2013Article
- Role of Scd5, a protein phosphatase-1 targeting protein, in phosphoregulation of Sla1 during endocytosis.Journal of cell science · 2012Article
- Molecular basis for coupling the plasma membrane to the actin cytoskeleton during clathrin-mediated endocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Lessons from yeast for clathrin-mediated endocytosis.Nature cell biology · 2011Review
- Yeast dynamin implicated in endocytic scission and the disassembly of endocytic components.Communicative & integrative biology · 2011Article
- Deciphering protein kinase specificity through large-scale analysis of yeast phosphorylation site motifs.Science signaling · 2010Article
- The yeast endocytic protein Epsin 2 functions in a cell-division signaling pathway.Journal of cell science · 2009Article
- Functions of actin in endocytosis.Cellular and molecular life sciences : CMLS · 2009Review
- A novel function of Arp2p in mediating Prk1p-specific regulation of actin and endocytosis in yeast.Molecular biology of the cell · 2008Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The yeast actin-regulating kinases Ark1p and Prk1p are signaling proteins localized to cortical actin patches, which may be sites of endocytosis. Interactions between the endocytic proteins Pan1p and End3p may be regulated by Prk1p-dependent threonine phosphorylation of Pan1p within the consensus sequence [L/I]xxQxTG. We identified two Prk1p phosphorylation sites within the Pan1p-binding protein Ent1p, a yeast epsin homologue, and demonstrate Prk1p-dependent phosphorylation of both threonines. Converting both threonines to either glutamate or alanine mimics constitutively phosphorylated or dephosphorylated Ent1p, respectively. Synthetic growth defects were observed in a pan1-20 ENT1(EE) double mutant, suggesting that Ent1p phosphorylation negatively regulates the formation/activity of a Pan1p-Ent1p complex. Interestingly, pan1-20 ent2 Delta but not pan1-20 ent1 Delta double mutants had improved growth and endocytosis over the pan1-20 mutant. We found that actin-regulating Ser/Thr kinase (ARK) mutants exhibit endocytic defects and that overexpressing either wild-type or alanine-substituted Ent1p partially suppressed phenotypes associated with loss of ARK kinases, including growth, endocytosis, and actin localization defects. Consistent with synthetic growth defects of pan1-20 ENT1(EE) cells, overexpressing glutamate-substituted Ent1p was deleterious to ARK mutants. Surprisingly, overexpressing the related Ent2p protein could not suppress ARK kinase mutant phenotypes. These results suggest that Ent1p and Ent2p are not completely redundant and may perform opposing functions in endocytosis. These data support the model that, as for clathrin-dependent recycling of synaptic vesicles, yeast endocytic protein phosphorylation inhibits endocytic functions.
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