ArticleMolecular biology of the cell2001
Cytoskeleton-dependent membrane domain segregation during neutrophil polarization.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
48 citing papers in PubMed, 123 citations in OpenAlex.
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- Extracellular Vesicles and Immune System Function: Exploring Novel Approaches to Colorectal Cancer Immunotherapy.Biomedicines · 2024Review
- Cholesterol Is a Regulator of CAV1 Localization and Cell Migration in Oral Squamous Cell Carcinoma.International journal of molecular sciences · 2023Article
- Hyaluronan promotes intracellular ROS production and apoptosis in TNFα-stimulated neutrophils.Frontiers in immunology · 2023Article
- Neutrophil-derived trail is a proinflammatory subtype of neutrophil-derived extracellular vesicles.Theranostics · 2021Article
- Immune Cell-Derived Extracellular Vesicles - New Strategies in Cancer Immunotherapy.Frontiers in immunology · 2021Review
- Do ABC transporters regulate plasma membrane organization?Cellular & molecular biology letters · 2020Review
- Leukocyte Cytoskeleton Polarization Is Initiated by Plasma Membrane Curvature from Cell Attachment.Developmental cell · 2019Article
- Review
- Cell surface topography is a regulator of molecular interactions during chemokine-induced neutrophil spreading.Biophysical journal · 2014Article
- Localization of phosphatidylinositol 4,5-bisphosphate to lipid rafts and uroids in the human protozoan parasite Entamoeba histolytica.Infection and immunity · 2013Article
- Cdc42 regulates neutrophil migration via crosstalk between WASp, CD11b, and microtubules.Blood · 2012Article
- Article
- Distribution of CD133 reveals glioma stem cells self-renew through symmetric and asymmetric cell divisions.Cell death & disease · 2011Article
- Dynamic reorganization of flotillins in chemokine-stimulated human T-lymphocytes.BMC cell biology · 2011Article
- Membrane raft organization is more sensitive to disruption by (n-3) PUFA than nonraft organization in EL4 and B cells.The Journal of nutrition · 2011Article
- Podoplanin associates with CD44 to promote directional cell migration.Molecular biology of the cell · 2010Article
- Flotillin microdomains interact with the cortical cytoskeleton to control uropod formation and neutrophil recruitment.The Journal of cell biology · 2010Article
- Migrating human neutrophils exhibit dynamic spatiotemporal variation in membrane lipid organization.American journal of respiratory cell and molecular biology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
On treatment with chemoattractant, the neutrophil plasma membrane becomes organized into detergent-resistant membrane domains (DRMs), the distribution of which is intimately correlated with cell polarization. Plasma membrane at the front of polarized cells is susceptible to extraction by cold Triton X-100, whereas membrane at the rear is resistant to extraction. After cold Triton X-100 extraction, DRM components, including the transmembrane proteins CD44 and CD43, the GPI-linked CD16, and the lipid analog, DiIC(16), are retained within uropods and cell bodies. Furthermore, CD44 and CD43 interact concomitantly with DRMs and with the F-actin cytoskeleton, suggesting a mechanism for the formation and stabilization of DRMs. By tracking the distribution of DRMs during polarization, we demonstrate that DRMs progress from a uniform distribution in unstimulated cells to small, discrete patches immediately after activation. Within 1 min, DRMs form a large cap comprising the cell body and uropod. This process is dependent on myosin in that an inhibitor of myosin light chain kinase can arrest DRM reorganization and cell polarization. Colabeling DRMs and F-actin revealed a correlation between DRM distribution and F-actin remodeling, suggesting that plasma membrane organization may orient signaling events that control cytoskeletal rearrangements and, consequently, cell polarity.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.