ArticleMolecular biology of the cell2001
pkl1(+)and klp2(+): Two kinesins of the Kar3 subfamily in fission yeast perform different functions in both mitosis and meiosis.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 116 citations in OpenAlex.
- Analyses of bent spindles reveal the mechanics of anaphase B in fission yeast.Molecular biology of the cell · 2026Article
- Analyses of bent spindles reveal the mechanics of anaphase B in fission yeast.bioRxiv : the preprint server for biology · 2025Article
- Cik1 and Vik1 accessory proteins confer distinct functions to the kinesin-14 Kar3.Journal of cell science · 2023Article
- The kinesin-5 protein Cut7 moves bidirectionally on fission yeast spindles with activity that increases in anaphase.Journal of cell science · 2023Article
- Kinesin Motors in the Filamentous Basidiomycetes in Light of theJournal of fungi (Basel, Switzerland) · 2022Review
- Force by minus-end motors Dhc1 and Klp2 collapses the S. pombe spindle after laser ablation.Biophysical journal · 2022Article
- The Putative RNA-Binding Protein Dri1 Promotes the Loading of Kinesin-14/Klp2 to the Mitotic Spindle and Is Sequestered into Heat-Induced Protein Aggregates in Fission Yeast.International journal of molecular sciences · 2021Article
- Tell the Difference Between Mitosis and Meiosis: Interplay Between Chromosomes, Cytoskeleton, and Cell Cycle Regulation.Frontiers in cell and developmental biology · 2021Review
- Klp2 and Ase1 synergize to maintain meiotic spindle stability during metaphase I.The Journal of biological chemistry · 2020Article
- Kinesin-14 family proteins and microtubule dynamics defineJournal of cell science · 2020Article
- Review
- Mechanisms of chromosome biorientation and bipolar spindle assembly analyzed by computational modeling.eLife · 2020Article
- Article
- CLASP promotes microtubule bundling in metaphase spindle independently of Ase1/PRC1 in fission yeast.Biology open · 2019Article
- Kinetochore-mediated outward force promotes spindle pole separation in fission yeast.Molecular biology of the cell · 2019Article
- Pivoting of microtubules driven by minus-end-directed motors leads to spindle assembly.BMC biology · 2019Article
- Kinesin-6 Klp9 plays motor-dependent and -independent roles in collaboration with Kinesin-5 Cut7 and the microtubule crosslinker Ase1 in fission yeast.Scientific reports · 2019Article
- Theory of Cytoskeletal Reorganization during Cross-Linker-Mediated Mitotic Spindle Assembly.Biophysical journal · 2019Article
- Suppressor Analysis Uncovers That MAPs and Microtubule Dynamics Balance with the Cut7/Kinesin-5 Motor for Mitotic Spindle Assembly inG3 (Bethesda, Md.) · 2019Article
- A microtubule polymerase cooperates with the kinesin-6 motor and a microtubule cross-linker to promote bipolar spindle assembly in the absence of kinesin-5 and kinesin-14 in fission yeast.Molecular biology of the cell · 2017Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
We have identified Klp2p, a new kinesin-like protein (KLP) of the KAR3 subfamily in fission yeast. The motor domain of this protein is 61% identical and 71% similar to Pkl1p, another fission yeast KAR3 protein, yet the two enzymes are different in behavior and function. Pkl1p is nuclear throughout the cell cycle, whereas Klp2p is cytoplasmic during interphase. During mitosis Klp2p enters the nucleus where it forms about six chromatin-associated dots. In metaphase-arrested cells these migrate back and forth across the nucleus. During early anaphase they segregate with the chromosomes into two sets of about three, fade, and are replaced by other dots that form on the spindle interzone. Neither klp2(+) nor pkl1(+) is essential, and the double deletion is also wild type for both vegetative and sexual reproduction. Each deletion rescues different alleles of cut7(ts), a KLP that contributes to spindle formation and elongation. When either or both deletions are combined with a dynein deletion, vegetative growth is normal, but sexual reproduction fails: klp2 Delta,dhc1-d1 in karyogamy, pkl1 Delta,dhc1-d1 in multiple phases of meiosis, and the triple deletion in both. Deletion of Klp2p elongates a metaphase-arrested spindle, but pkl1 Delta shortens it. The anaphase spindle of klp2 Delta becomes longer than the cell, leading it to curl around the cell's ends. Apparently, Klp2p promotes spindle disassembly and contributes to the behavior of mitotic chromosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.