ArticleMolecular biology of the cell2001
Increased levels of keratin 16 alter epithelialization potential of mouse skin keratinocytes in vivo and ex vivo.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 70 citations in OpenAlex.
- Pink lotus flower (Biomedical reports · 2026Article
- Insights into Keratinocyte and Immunologic Landscape in Cutaneous Graft-Versus-Host Disease through Single-Cell Transcriptomics.JID innovations : skin science from molecules to population health · 2025Article
- Expression Distribution of Keratins in Normal and Pathological Corneas and the Regulatory Role of Krt17 on Limbal Stem Cells.Investigative ophthalmology & visual science · 2025Article
- CD4 expression controls epidermal stem cell balance.Scientific reports · 2025Article
- Sulforaphane alleviates psoriasis by enhancing antioxidant defense through KEAP1-NRF2 Pathway activation and attenuating inflammatory signaling.Cell death & disease · 2023Article
- Antioxidant Capacity ofPlants (Basel, Switzerland) · 2022Article
- miR-204-3p downregulates KRT16 and promotes corneal repair in tree shrew fungal keratitis model.American journal of translational research · 2022Article
- Emerging Insights into Keratin 16 Expression during Metastatic Progression of Breast Cancer.Cancers · 2021Article
- Defining Epidermal Basal Cell States during Skin Homeostasis and Wound Healing Using Single-Cell Transcriptomics.Cell reports · 2020Article
- Regulation of keratin network dynamics by the mechanical properties of the environment in migrating cells.Scientific reports · 2020Article
- N-Terminal Acetylation and C-Terminal Amidation ofMarine drugs · 2019Article
- Review
- Microtubule-associated protein 4 phosphorylation regulates epidermal keratinocyte migration and proliferation.International journal of biological sciences · 2019Article
- Article
- Keratin 6 regulates collective keratinocyte migration by altering cell-cell and cell-matrix adhesion.The Journal of cell biology · 2018Article
- DNA polymerase ζ deficiency causes impaired wound healing and stress-induced skin pigmentation.Life science alliance · 2018Article
- Comparison of advanced therapy medicinal product gingiva and skin substitutes and their in vitro wound healing potentials.Journal of tissue engineering and regenerative medicine · 2018Article
- STAT6 Mediates Footpad Immunopathology in the Absence of IL-12p40 Following Infection of Susceptible BALB/c Mice WithFrontiers in immunology · 2018Article
- Epidermal Overexpression of Xenobiotic Receptor PXR Impairs the Epidermal Barrier and Triggers Th2 Immune Response.The Journal of investigative dermatology · 2018Article
- Intermediate Filaments and the Regulation of Cell Motility during Regeneration and Wound Healing.Cold Spring Harbor perspectives in biology · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The process of wound repair in adult skin is complex, involving dermal contraction and epithelial migration to repair the lesion and restore the skin's barrier properties. At the wound edge, keratinocytes undergo many changes that engender an epithelialization behavior. The type II keratin 6 and type I keratins 16 and 17 are induced well before cell migration begins, but the role of these proteins is not understood. Forced expression of human K16 in skin epithelia of transgenic mice has been shown to cause dose-dependent skin lesions concomitant with alterations in keratin filament organization and in cell adhesion. Here we show, with the use of a quantitative assay, that these transgenic mice show a delay in the closure of full-thickness skin wounds in situ compared with wild-type and low-expressing K16 transgenic mice. We adapted and validated an ex vivo skin explant culture system to better assess epithelialization in a wound-like environment. Transgenic K16 explants exhibit a significant reduction of keratinocyte outgrowth in this setting. This delay is transgene dose-dependent, and is more severe when K16 is expressed in mitotic compared with post-mitotic keratinocytes. Various lines of evidence suggest that the mechanism(s) involved is complex and not strictly cell autonomous. These findings have important implications for the function of K16 in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.