ArticleJournal of anatomy2001
The differential distribution of acetylated and detyrosinated alpha-tubulin in the microtubular cytoskeleton and primary cilia of hyaline cartilage chondrocytes.
Article in Journal of anatomy, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 97 citations in OpenAlex.
- LIPUS regulates the progression of knee osteoarthritis in mice through primary cilia-mediated TRPV4 channels.Apoptosis : an international journal on programmed cell death · 2024Article
- HDAC6 inhibition regulates substrate stiffness-mediated inflammation signaling in chondrocytes.Acta biochimica et biophysica Sinica · 2023Article
- Primary cilium-mediated mechanotransduction in cartilage chondrocytes.Experimental biology and medicine (Maywood, N.J.) · 2023Review
- Chondrocyte primary cilia lengthening and shortening in response to mediators of osteoarthritis; a role for integrin α1β1 and focal adhesions.Osteoarthritis and cartilage open · 2023Article
- The Primary Cilium on Cells of Developing Skeletal Rudiments; Distribution, Characteristics and Response to Mechanical Stimulation.Frontiers in cell and developmental biology · 2021Article
- Primary cilia: Versatile regulator in cartilage development.Cell proliferation · 2020Review
- Galectin 3 Deficiency Alters Chondrocyte Primary Cilium Formation and Exacerbates Cartilage Destruction via Mitochondrial Apoptosis.International journal of molecular sciences · 2020Article
- The primary cilium as a signaling nexus for growth plate function and subsequent skeletal development.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2018Review
- Osteoarthritis-Like Changes in Bardet-Biedl Syndrome Mutant Ciliopathy Mice (Frontiers in physiology · 2018Article
- Biological and Chemical Removal of Primary Cilia Affects Mechanical Activation of Chondrogenesis Markers in Chondroprogenitors and Hypertrophic Chondrocytes.International journal of molecular sciences · 2016Article
- The role of hedgehog signalling in skeletal health and disease.Nature reviews. Rheumatology · 2015Review
- The growth plate's response to load is partially mediated by mechano-sensing via the chondrocytic primary cilium.Cellular and molecular life sciences : CMLS · 2015Article
- Primary cilia exist in a small fraction of cells in trabecular bone and marrow.Calcified tissue international · 2015Article
- Function and regulation of primary cilia and intraflagellar transport proteins in the skeleton.Annals of the New York Academy of Sciences · 2015Review
- Effects of α-tubulin K40 acetylation and detyrosination on kinesin-1 motility in a purified system.Biophysical journal · 2014Article
- An experimental and computational analysis of primary cilia deflection under fluid flow.Computer methods in biomechanics and biomedical engineering · 2014Article
- The primary cilium as a dual sensor of mechanochemical signals in chondrocytes.Cellular and molecular life sciences : CMLS · 2012Review
- Primary cilia mediate mechanotransduction through control of ATP-induced Ca2+ signaling in compressed chondrocytes.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- Depletion of primary cilia in articular chondrocytes results in reduced Gli3 repressor to activator ratio, increased Hedgehog signaling, and symptoms of early osteoarthritis.Osteoarthritis and cartilage · 2012Article
- The mechanics of the primary cilium: an intricate structure with complex function.Journal of biomechanics · 2012Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The primary cilium is a ubiquitous cytoplasmic organelle of unknown function. Ultrastructural evidence of primary cilia in chondrocytes, and their colocalisation with the Golgi apparatus, has led to speculation that these structures are functionally linked. To investigate the relationship between these organelles, we examined the molecular anatomy of the microtubular cytoskeleton in the chondrocytes of chick embryo sterna. Thick cryosections were immunolabelled with antibodies directed against acetylated alpha-tubulin (C3B9), detyrosinated alpha-tubulin (ID5) and total alpha-tubulin (TAT), and imaged at high magnification using confocal laser scanning microscopy. Transmission electron microscopy confirmed the ultrastructure of the chondrocyte primary cilium and its structural relationship to the Golgi apparatus. Detyrosinated and acetylated alpha-tubulins were concentrated in the centrioles, centrosome and microtubule organising centre adjacent to the nucleus, with total alpha-tubulin distributed throughout the cytoplasm. ID5 stained the primary cilium at an incidence of 1 per cell, its colocalisation with C3B9 identifying the primary cilium as one of the most stable features of the microtubular cytoskeleton. Primary cilia varied from 1 to 4 microm in length, and 3 patterns of projection into the extracellular matrix were identified; (1) full extension and matrix contact, with minor undulations along the length; (2) partial extension and matrix contact, with a range of bending deflections; (3) cilium reclined against the cell surface with minimal matrix contact. Ultrastructural studies identified direct connections between extracellular collagen fibres and the proteins which decorate ciliary microtubules, suggesting a matrix-cilium-Golgi continuum in hyaline chondrocytes. These results strengthen the hypothesis that the primary cilium acts as a 'cellular cybernetic probe' capable of transducing environmental information from the extracellular matrix, communicating this information to the centrosome. and regulating the exocytosis of Golgi-derived secretory vesicles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.