ReviewMolecular and cellular biochemistry2001
Improving the quality of industrially important enzymes by directed evolution.
Review in Molecular and cellular biochemistry, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- Breaking down microplastics: insights into the role of actinomycetes in biotic degradation pathways.Archives of microbiology · 2025Review
- Directed Evolution of Xylanase fromPolymers · 2025Article
- Exploring the Stability and Substrate Profile of Transaminase from Silicibacter pomeroyi with Ancestral Sequence Reconstruction.Chembiochem : a European journal of chemical biology · 2025Article
- Development of specialized devices for microbial experimental evolution.Development, growth & differentiation · 2024Review
- An overview of three biocatalysts of pharmaceutical importance synthesized by microbial cultures.AIMS microbiology · 2021Review
- Isolation and Characterization of Thermophilic Bacteria from Jordanian Hot Springs:International journal of microbiology · 2017Article
- Fungal Beta-glucosidases: a bottleneck in industrial use of lignocellulosic materials.Biomolecules · 2013Article
- Microbial enzymes with special characteristics for biotechnological applications.Biomolecules · 2013Article
- Thermostable DNA ligase-mediated PCR production of circular plasmid (PPCP) and its application in directed evolution via in situ error-prone PCR.DNA research : an international journal for rapid publication of reports on genes and genomes · 2013Article
- A thermostable β-glucuronidase obtained by directed evolution as a reporter gene in transgenic plants.PloS one · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Directed evolution is a new process for developing industrially viable biocatalysts. This technique does not require a comprehensive knowledge of the relationships between sequence structure and function of proteins as required by protein engineering. It mimics the process of Darwinian evolution in a test tube combining random mutagenesis and recombination with screening or selection for enzyme variants that have the desired properties. Directed evolution helps in enhancing the enzyme performance both in natural and synthetic environments. This article reviews the process of directed evolution and its application to improve substrate specificity, activity, enantioselectivity and thermal stability.
Indexed as
Identifiers
11693193What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.