ArticleNucleic acids research2001
Robust and efficient synthetic method for forming DNA microarrays.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Integrated Affinity Biosensing Platforms on Screen-Printed Electrodes Electrografted with Diazonium Salts.Sensors (Basel, Switzerland) · 2018Review
- Reversible DNA micro-patterning using the fluorous effect.Chemical communications (Cambridge, England) · 2017Article
- Photo-attachment of biomolecules for miniaturization on wicking Si-nanowire platform.PloS one · 2015Article
- Immobilization techniques for microarray: challenges and applications.Sensors (Basel, Switzerland) · 2014Review
- Article
- An efficient covalent coating on glass slides for preparation of optical oligonucleotide microarrays.Iranian journal of basic medical sciences · 2013Article
- Development of polymer-coated glass slides as optical oligonucleotide microarrays.Avicenna journal of medical biotechnology · 2013Article
- Facile DNA immobilization on surfaces through a catecholamine polymer.Angewandte Chemie (International ed. in English) · 2011Article
- Stripping custom microRNA microarrays and the lessons learned about probe-slide interactions.Analytical biochemistry · 2009Article
- Development and application of a microarray meter tool to optimize microarray experiments.BMC research notes · 2008Article
- Robert L. Metzenberg, June 11, 1930-July 15, 2007: geneticist extraordinaire and "model human".Genetics · 2008Article
- Increasing the sensitivity of DNA microarrays by metal-enhanced fluorescence using surface-bound silver nanoparticles.Nucleic acids research · 2007Article
- Multiple primer extension by DNA polymerase on a novel plastic DNA array coated with a biocompatible polymer.Nucleic acids research · 2007Article
- Microcoding: the second step in DNA barcoding.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2005Review
- Microarray analysis of Escherichia coli O157:H7.World journal of gastroenterology · 2005Article
- An Activated GOPS-poly-Engineering in life sciences · 2005Article
- Optimization of gold nanoparticle-based DNA detection for microarrays.Journal of fluorescence · 2005Article
- Development and assessment of microarray-based DNA fingerprinting in Eucalyptus grandis.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2004Article
- Synergistic effects of epoxy- and amine-silanes on microarray DNA immobilization and hybridization.The Biochemical journal · 2003Article
- Impact of surface chemistry and blocking strategies on DNA microarrays.Nucleic acids research · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The field of DNA microarray technology has necessitated the cooperative efforts of interdisciplinary scientific teams to achieve its primary goal of rapidly measuring global gene expression patterns. A collaborative effort was established to produce a chemically reactive surface on glass slide substrates to which unmodified DNA will covalently bind for improvement of cDNA microarray technology. Using the p-aminophenyl trimethoxysilane (ATMS)/diazotization chemistry that was developed, microarrays were fabricated and analyzed. This immobilization method produced uniform spots containing equivalent or greater amounts of DNA than commercially available immobilization techniques. In addition, hybridization analyses of microarrays made with ATMS/diazotization chemistry showed very sensitive detection of the target sequence, two to three orders of magnitude more sensitive than the commercial chemistries. Repeated stripping and re-hybridization of these slides showed that DNA loss was minimal, allowing multiple rounds of hybridization. Thus, the ATMS/diazotization chemistry facilitated covalent binding of unmodified DNA, and the reusable microarrays that were produced showed enhanced levels of hybridization and very low background fluorescence.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.