ArticleNucleic acids research2001
DNA sequencing using biotinylated dideoxynucleotides and mass spectrometry.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- An integrated system for DNA sequencing by synthesis using novel nucleotide analogues.Accounts of chemical research · 2010Article
- Four-color DNA sequencing with 3'-O-modified nucleotide reversible terminators and chemically cleavable fluorescent dideoxynucleotides.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- 3'-O-modified nucleotides as reversible terminators for pyrosequencing.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- The involvement of replication in single stranded oligonucleotide-mediated gene repair.Nucleic acids research · 2006Article
- Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Design and synthesis of a 3'-O-allyl photocleavable fluorescent nucleotide as a reversible terminator for DNA sequencing by synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Digital detection of genetic mutations using SPC-sequencing.Genome research · 2004Article
- Microfluidic device reads up to four consecutive base pairs in DNA sequencing-by-synthesis.Nucleic acids research · 2004Article
- Design and synthesis of a photocleavable biotinylated nucleotide for DNA analysis by mass spectrometry.Nucleic acids research · 2004Article
- Photocleavage of a 2-nitrobenzyl linker bridging a fluorophore to the 5' end of DNA.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- A photocleavable fluorescent nucleotide for DNA sequencing and analysis.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Solid phase capturable dideoxynucleotides for multiplex genotyping using mass spectrometry.Nucleic acids research · 2002Article
- Single nucleotide polymorphism detection by combinatorial fluorescence energy transfer tags and biotinylated dideoxynucleotides.Nucleic acids research · 2002Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MS) has been explored widely for DNA sequencing. The major requirement for this method is that the DNA sequencing fragments must be free from alkaline and alkaline earth salts as well as other contaminants for accurately measuring the masses of the DNA fragments. We report here the development of a novel MS DNA sequencing method that generates Sanger-sequencing fragments in one tube using biotinylated dideoxynucleotides. The DNA sequencing fragments that carry a biotin at the 3'-end are made free from salts and other components in the sequencing reaction by capture with streptavidin-coated magnetic beads. Only correctly terminated biotinylated DNA fragments are subsequently released and loaded onto a mass spectrometer to obtain accurate DNA sequencing data. Compared with gel electrophoresis-based sequencing systems, MS produces a very high resolution of DNA-sequencing fragments, fast separation on microsecond time scales, and completely eliminates the compressions associated with gel electrophoresis. The high resolution of MS allows accurate mutation and heterozygote detection. This optimized solid-phase DNA-sequencing chemistry plus future improvements in detector sensitivity for large DNA fragments in MS instrumentation will further improve MS for DNA sequencing.
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