ArticleNucleic acids research2001
5-Methylcytosine DNA glycosylase participates in the genome-wide loss of DNA methylation occurring during mouse myoblast differentiation.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 94 citations in OpenAlex.
- The DNA repair function of BCL11A suppresses senescence and promotes continued proliferation of triple-negative breast cancer cells.NAR cancer · 2022Article
- DNA methylation dynamics of genomic imprinting in mouse development.Biology of reproduction · 2018Review
- Recent advances in mammalian protein production.FEBS letters · 2014Review
- Review
- Advances in Mammalian cell line development technologies for recombinant protein production.Pharmaceuticals (Basel, Switzerland) · 2013Article
- DNA demethylation pathways: recent insights.Genetics & epigenetics · 2013Review
- Article
- Modulation of cell cycle progression by 5-azacytidine is associated with early myogenesis induction in murine myoblasts.International journal of biological sciences · 2013Article
- Amniotic membrane-derived cells inhibit proliferation of cancer cell lines by inducing cell cycle arrest.Journal of cellular and molecular medicine · 2012Article
- APOBEC2 is a monomer in solution: implications for APOBEC3G models.Biochemistry · 2012Article
- Article
- Role of gene methylation in antitumor immune response: implication for tumor progression.Cancers · 2011Article
- Parole terms for a killer: directing caspase3/CAD induced DNA strand breaks to coordinate changes in gene expression.Cell cycle (Georgetown, Tex.) · 2010Article
- Brain function and chromatin plasticity.Nature · 2010Review
- DNA hypomethylation in cancer cells.Epigenomics · 2009Review
- DNA excision repair proteins and Gadd45 as molecular players for active DNA demethylation.Cell cycle (Georgetown, Tex.) · 2009Review
- Epigenetics in acute myeloid leukemia.Seminars in oncology · 2008Review
- Massively parallel bisulphite pyrosequencing reveals the molecular complexity of breast cancer-associated cytosine-methylation patterns obtained from tissue and serum DNA.Genome research · 2008Article
- Active cytosine demethylation triggered by a nuclear receptor involves DNA strand breaks.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- DEMETER and REPRESSOR OF SILENCING 1 encode 5-methylcytosine DNA glycosylases.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
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7 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Changes in gene expression during mouse myoblast differentiation were monitored by DNA microarray hybridisation. Four days after the onset of differentiation 2.37% of the genes increased in activity from a value of zero, whereas during the same time 1.68% of total genes had decreased expression. During the first 24 h of differentiation an average of 700 000 CpG sites per haploid genome were demethylated. Maximal loss of DNA methylation is attained after 2 days of differentiation, followed by a gradual remethylation. The highest demethylation is observed in highly repeated DNA sequences, followed by single copy sequences. When DNA replication is inhibited by aphidicolin or L-mimosine this genome-wide demethylation is still observed. During the first 3 h of differentiation there is an increase in the number of hemimethylated CpG sites, which disappear rapidly during the course of genome-wide hypomethylation. Transfection of cells with an antisense morpholino oligonucleotide to 5-methylcytosine DNA glycosylase (G/T mismatch DNA glycosylase) decreases both the activity of the enzyme and genome-wide demethylation. It is concluded that the genome-wide loss of DNA methylation in differentiating mouse myoblasts occurs in part by formation of hemimethylated CpG sites, which can serve as the substrate for 5-methylcytosine-DNA glycosylase.
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