ArticleNucleic acids research2001
Replication intermediate analysis confirms that chromosomal replication origin initiates from an unusual intergenic region in Caulobacter crescentus.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- Computational modeling of unphosphorylated CtrA:iScience · 2021Article
- Selective sequestration of signalling proteins in a membraneless organelle reinforces the spatial regulation of asymmetry in Caulobacter crescentus.Nature microbiology · 2020Article
- Comparison of the complete genome sequences of four γ-hexachlorocyclohexane-degrading bacterial strains: insights into the evolution of bacteria able to degrade a recalcitrant man-made pesticide.DNA research : an international journal for rapid publication of reports on genes and genomes · 2016Article
- Replication initiator DnaA binds at the Caulobacter centromere and enables chromosome segregation.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Getting in the loop: regulation of development in Caulobacter crescentus.Microbiology and molecular biology reviews : MMBR · 2010Review
- Complex regulatory pathways coordinate cell-cycle progression and development in Caulobacter crescentus.Advances in microbial physiology · 2009Review
- Ubiquitous expression of mRFP-1 in vivo by site-directed transgenesis.Transgenic research · 2007Article
- Analysis of the terminus region of the Caulobacter crescentus chromosome and identification of the dif site.Journal of bacteriology · 2006Article
- The obligate human pathogen, Neisseria gonorrhoeae, is polyploid.PLoS biology · 2006Article
- Conserved response regulator CtrA and IHF binding sites in the alpha-proteobacteria Caulobacter crescentus and Rickettsia prowazekii chromosomal replication origins.Journal of bacteriology · 2002Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The alpha-proteobacterium Caulobacter crescentus possesses a developmental cell cycle that restricts chromosome replication to a stalked cell type. The proposed C.crescentus chromosome replication origin (Cori) lies between hemE and RP001, an unusual intergenic region not previously associated with bacterial replication origins, although a similar genomic arrangement is also present at the putative replication origin in the related bacterium Rickettsia prowazekii. The cloned Cori supports autonomous plasmid replication selectively in the stalked cell type implying that replication of the entire chromosome also initiates between hemE and RP001. To confirm this location, we applied the 2-D (N/N) agarose gel electrophoresis technique to resolve and identify chromosome replication intermediates throughout a 30 kb region spanning Cori. Replication initiation in Cori was uniquely characterized by an 'origin bubble and Y-arc' pattern and this observation was supported by simple replication fork 'Y-arc' patterns that characterized the regions flanking Cori. These replication forks originated bi-directionally from within Cori as determined by the fork direction assay. Therefore, chromosomal replication initiates from the unusual hemE/RP001 intergenic region that we propose represents a new class of replication origins.
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