ArticleNucleic acids research2001
Transgene-mediated post-transcriptional gene silencing is inhibited by 3' non-coding sequences in Paramecium.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 39 citations in OpenAlex.
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- TFIIS-Dependent Non-coding Transcription Regulates Developmental Genome Rearrangements.PLoS genetics · 2015Article
- Primary and secondary siRNA synthesis triggered by RNAs from food bacteria in the ciliate Paramecium tetraurelia.Nucleic acids research · 2015Article
- Genome-wide analysis of genetic and epigenetic control of programmed DNA deletion.Nucleic acids research · 2014Article
- A forward genetic screen reveals essential and non-essential RNAi factors in Paramecium tetraurelia.Nucleic acids research · 2014Article
- Pdsg1 and Pdsg2, novel proteins involved in developmental genome remodelling in Paramecium.PloS one · 2014Article
- Epigenetics of ciliates.Cold Spring Harbor perspectives in biology · 2013Review
- Functional specialization of Piwi proteins in Paramecium tetraurelia from post-transcriptional gene silencing to genome remodelling.Nucleic acids research · 2011Article
- RNA-Mediated Epigenetic Programming of Genome Rearrangements.Annual review of genomics and human genetics · 2011Review
- Distinct RNA-dependent RNA polymerases are required for RNAi triggered by double-stranded RNA versus truncated transgenes in Paramecium tetraurelia.Nucleic acids research · 2010Article
- Epigenetic inheritance in ciliates.Current opinion in microbiology · 2009Review
- Silencing-associated and meiosis-specific small RNA pathways in Paramecium tetraurelia.Nucleic acids research · 2009Article
- Maternal noncoding transcripts antagonize the targeting of DNA elimination by scanRNAs in Paramecium tetraurelia.Genes & development · 2008Article
- Induction of gene silencing by hairpin RNA expression in Tetrahymena thermophila reveals a second small RNA pathway.Molecular and cellular biology · 2006Article
- Epigenetic control of chromosome breakage at the 5' end of Paramecium tetraurelia gene A.Eukaryotic cell · 2004Article
- RNA-mediated programming of developmental genome rearrangements in Paramecium tetraurelia.Molecular and cellular biology · 2004Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Homology-dependent gene silencing is achieved in Paramecium by introduction of gene coding regions into the somatic nucleus at high copy number, resulting in reduced expression of all homologous genes. Although a powerful tool for functional analysis, the relationship of this phenomenon to gene silencing mechanisms in other organisms has remained obscure. We report here experiments using the T4a gene, a member of the trichocyst [corrected]matrix protein (TMP) multigene family encoding secretory proteins, and the ND7 gene, a single copy gene required for exocytotic membrane fusion. Silencing of either gene leads to an exocytosis-deficient phenotype easily scored on individual cells. For each gene we have tested the ability of different combinations of promoter, coding and 3' non-coding regions to provoke silencing, and analyzed transcription and steady-state RNA in the transformed cells. We provide evidence that homology-dependent gene silencing in Paramecium is post-transcriptional and that both sense and antisense RNA are transcribed from the transgenes, consistent with a role for dsRNA in triggering silencing. Constructs with and without promoters induce gene silencing. However, transgenes that contain 3' non-coding regions do not induce gene silencing, despite antisense RNA production. We present a model according to which different pathways of RNA metabolism compete for transcripts and propose that the relative efficiencies of dsRNA formation and of 3' RNA processing of sense transgene transcripts determine the outcome of transformation experiments.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.