ArticleThe Journal of physiology2001
ACh-induced rebound stimulation of L-type Ca(2+) current in guinea-pig ventricular myocytes, mediated by Gbetagamma-dependent activation of adenylyl cyclase.
Article in The Journal of physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Autonomic imbalance in cardiovascular disease: molecular mechanisms and emerging therapeutics.American journal of physiology. Cell physiology · 2025Review
- Nonlinear high-activity neuronal excitation enhances odor discrimination.Current biology : CB · 2025Article
- Nanodomain cAMP signaling in cardiac pathophysiology: potential for developing targeted therapeutic interventions.Physiological reviews · 2025Review
- Compartmentalized cAMP signaling in cardiac ventricular myocytes.Cellular signalling · 2022Review
- Mechanisms of cAMP compartmentation in cardiac myocytes: experimental and computational approaches to understanding.The Journal of physiology · 2021Review
- The Roles of Cardiovascular HFrontiers in pharmacology · 2021Review
- Mechanisms Underlying the Cardioprotection of YangXinDingJi Capsule against Myocardial Ischemia in Rats.Evidence-based complementary and alternative medicine : eCAM · 2020Article
- A multiscale computational modelling approach predicts mechanisms of female sex risk in the setting of arousal-induced arrhythmias.The Journal of physiology · 2017Article
- Cardiac inotropic rebound effect after washout of acetylcholine is associated with electrophysiological heterogeneity in Langendorff-perfused rabbit heart.Experimental and therapeutic medicine · 2014Article
- Mechanisms of cyclic AMP compartmentation revealed by computational models.The Journal of general physiology · 2014Article
- Caveolae create local signalling domains through their distinct protein content, lipid profile and morphology.Journal of molecular and cellular cardiology · 2012Review
- Regulation by Ca2+-signaling pathways of adenylyl cyclases.Cold Spring Harbor perspectives in biology · 2011Review
- Cytoplasmic cAMP concentrations in intact cardiac myocytes.American journal of physiology. Cell physiology · 2008Article
- Nuclear localization of Myomesin-1: possible functions.Journal of muscle research and cell motility · 2008Article
- Ca2+-stimulated adenylyl cyclase isoform AC1 is preferentially expressed in guinea-pig sino-atrial node cells and modulates the I(f) pacemaker current.The Journal of physiology · 2007Article
- cAMP microdomains and L-type Ca2+ channel regulation in guinea-pig ventricular myocytes.The Journal of physiology · 2007Article
- Compartmentation of cAMP signaling in cardiac myocytes: a computational study.Biophysical journal · 2007Article
- Pacemaker channels in mouse thalamocortical neurones are regulated by distinct pathways of cAMP synthesis.The Journal of physiology · 2004Article
- Muscarinic regulation of cardiac ion channels.British journal of pharmacology · 2003Review
- Cholinergic modulation of the basal L-type calcium current in ferret right ventricular myocytes.The Journal of physiology · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
1. The effects that muscarinic receptor stimulation have on the cAMP-dependent regulation of L-type Ca(2+) currents were studied in isolated guinea-pig ventricular myocytes using the whole-cell configuration of the patch-clamp technique. 2. The muscarinic agonist ACh inhibited the Ca(2+) current stimulated by the beta-adrenergic agonist isoprenaline (Iso), and washout of ACh revealed a stimulatory response that appeared as a transient rebound increase in the amplitude of the Ca(2+) current. The ACh-induced stimulatory effect was not observed in the absence of Iso. 3. ACh-induced rebound stimulation was also observed in the presence of H(2) histamine receptor activation and cholera toxin treatment, which like beta-adrenergic receptor activation enhance adenylyl cyclase (AC) activity in a stimulatory G protein (G(s))-dependent manner. ACh-induced rebound stimulation was not observed in the presence of forskolin, which enhances AC activity in a G(s)-independent manner. 4. Pertussis toxin (PTX) treatment blocked both the stimulatory and inhibitory effects of ACh. Intracellular dialysis with QEHA, a peptide that binds free G protein betagamma subunits, selectively antagonized the stimulatory effect, leaving an enhanced inhibitory effect. 5. Evidence for the expression of AC4, an isoform of AC that can be stimulated by Gbetagamma but only in the presence of Galpha(s), was obtained by Western blot analysis of guinea-pig ventricular myocyte membrane preparations. 6. These results suggest that muscarinic receptor stimulation facilitates as well as inhibits cAMP-dependent regulation of the Ca(2+) current and that the net response is a balance between these two actions. We suggest that the stimulatory effect is due to a direct activation of AC4 by the betagamma subunits of a PTX-sensitive G protein.
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