ArticleBMC microbiology2001
Lactoferrin and free secretory component of human milk inhibit the adhesion of enteropathogenic Escherichia coli to HeLa cells.
Article in BMC microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 69 citations in OpenAlex.
- Maternal milk contains antimicrobial factors that protect young rabbits from enteropathogenic Escherichia coli infection.Clinical and vaccine immunology : CVI · 2007Trial
- Lactoferrin impairs pathogen virulence through its proteolytic activity.Frontiers in veterinary science · 2024Review
- The Role of Lactoferrin in Intestinal Health.Pharmaceutics · 2023Review
- Diverse Mechanisms of Antimicrobial Activities of Lactoferrins, Lactoferricins, and Other Lactoferrin-Derived Peptides.International journal of molecular sciences · 2021Review
- Active free secretory component and secretory IgA in human milk: do maternal vaccination, allergy, infection, mode of delivery, nutrition and active lifestyle change their concentrations?Pediatric research · 2021Article
- Lactoferrin and Its Derived Peptides: An Alternative for Combating Virulence Mechanisms Developed by Pathogens.Molecules (Basel, Switzerland) · 2020Review
- Augmentation of Urinary Lactoferrin Enhances Host Innate Immune Clearance of Uropathogenic Escherichia coli.Journal of innate immunity · 2019Article
- Lactoferrin: A Natural Glycoprotein Involved in Iron and Inflammatory Homeostasis.International journal of molecular sciences · 2017Review
- Adherence inhibition of Cronobacter sakazakii to intestinal epithelial cells by lactoferrin.Current microbiology · 2014Article
- Effect of hyperoxia on pulmonary SIgA and its components, IgA and SC.Journal of clinical immunology · 2013Article
- Polymeric immunoglobulin receptor traffics through two distinct apically targeted pathways in primary lacrimal gland acinar cells.Journal of cell science · 2013Article
- Adhesion of Diarrheagenic Escherichia coli and Inhibition by Glycocompounds Engaged in the Mucosal Innate Immunity.Biology · 2013Article
- Lactoferrin and necrotizing enterocolitis.Clinics in perinatology · 2013Review
- Mini-review: Lactoferrin: a bioinspired, anti-biofilm therapeutic.Biofouling · 2013Review
- Effect of lactoferrin on enteric pathogens.Biochimie · 2009Review
- In vitro adhesion and invasion inhibition of Shigella dysenteriae, Shigella flexneri and Shigella sonnei clinical strains by human milk proteins.BMC microbiology · 2004Article
- Lactoferrin impairs type III secretory system function in enteropathogenic Escherichia coli.Infection and immunity · 2003Article
- Lactoferrin protects rabbits from Shigella flexneri-induced inflammatory enteritis.Infection and immunity · 2002Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDiarrhoea caused by Escherichia coli is an important cause of infant morbidity and mortality in developing countries. Enteropathogenic Escherichia coli (EPEC) is considered one of the major causes of diarrhoea in children living in developing countries. The ability of diarrhoeagenic strains of E. coli to adhere to and colonize the intestine is the first step towards developing the disease. EPEC strains adhere to enterocytes and HeLa cells in a characteristic pattern known as localized adherence. Many epidemiological studies of diarrhoea have shown that breast-feeding protects infants from intestinal infections. Both immunoglobulin and non-immunoglobulin elements of human milk are thought to contribute to the protection from diarrhoeal agents.
resultsThe effects of human milk and its protein components on the localized adherence of EPEC were investigated. Non-immunoglobulin components of human milk responsible for the inhibition of EPEC adhesion to HeLa cells were isolated by chromatographic fractionation of human whey proteins. Besides secretory immunoglobulin A, which has been previously reported to affect the adhesion of EPEC, free secretory component (fSC) and lactoferrin (Lf) were isolated. Even in concentrations lower than those usually found in whole milk, fSC and Lf were able to inhibit the adhesion of EPEC. alpha-lactalbumin was also isolated, but showed no activity on EPEC adhesion.
conclusionsThis study demonstrated that the immunoglobulin fraction, the free secretory component and lactoferrin of human milk inhibit EPEC adhesion to HeLa cells. These results indicate that fSC and Lf may be important non-specific defence factors against EPEC infections.
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