ArticleMolecular and cellular biology2001
Telomere formation by rap1p binding site arrays reveals end-specific length regulation requirements and active telomeric recombination.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Rap1-mediated steric hindrance protects telomeres from MRX sensing.Nature structural & molecular biology · 2026Article
- Article
- A new assay capturing chromosome fusions shows a protection trade-off at telomeres and NHEJ vulnerability to low-density ionizing radiation.Nucleic acids research · 2021Article
- A sharp Pif1-dependent threshold separates DNA double-strand breaks from critically short telomeres.eLife · 2017Article
- Endogenous Hot Spots of De Novo Telomere Addition in the Yeast Genome Contain Proximal Enhancers That Bind Cdc13.Molecular and cellular biology · 2016Article
- Pif1 removes a Rap1-dependent barrier to the strand displacement activity of DNA polymerase δ.Nucleic acids research · 2016Article
- Review
- Novel transcript truncating function of Rap1p revealed by synthetic codon-optimized Ty1 retrotransposon.Genetics · 2012Article
- Characterization of the yeast telomere nucleoprotein core: Rap1 binds independently to each recognition site.The Journal of biological chemistry · 2010Article
- Dicentric breakage at telomere fusions.Genes & development · 2010Article
- Telomere length regulation: coupling DNA end processing to feedback regulation of telomerase.The EMBO journal · 2009Review
- Mutant telomeric repeats in yeast can disrupt the negative regulation of recombination-mediated telomere maintenance and create an alternative lengthening of telomeres-like phenotype.Molecular and cellular biology · 2009Article
- DNA breaks are masked by multiple Rap1 binding in yeast: implications for telomere capping and telomerase regulation.Genes & development · 2007Article
- Rap1 prevents telomere fusions by nonhomologous end joining.The EMBO journal · 2005Article
- The telomerase cycle: normal and pathological aspects.Journal of molecular medicine (Berlin, Germany) · 2005Review
- Counting of Rif1p and Rif2p on Saccharomyces cerevisiae telomeres regulates telomere length.Molecular and cellular biology · 2004Article
- Pol12, the B subunit of DNA polymerase alpha, functions in both telomere capping and length regulation.Genes & development · 2004Article
- Genetic dissection of the Kluyveromyces lactis telomere and evidence for telomere capping defects in TER1 mutants with long telomeres.Eukaryotic cell · 2004Article
- The number of vertebrate repeats can be regulated at yeast telomeres by Rap1-independent mechanisms.The EMBO journal · 2003Article
- The different (sur)faces of Rap1p.Molecular genetics and genomics : MGG · 2003Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rap1p, the major telomere repeat binding protein in yeast, has been implicated in both de novo telomere formation and telomere length regulation. To characterize the role of Rap1p in these processes in more detail, we studied the generation of telomeres in vivo from linear DNA substrates containing defined arrays of Rap1p binding sites. Consistent with previous work, our results indicate that synthetic Rap1p binding sites within the internal half of a telomeric array are recognized as an integral part of the telomere complex in an orientation-independent manner that is largely insensitive to the precise spacing between adjacent sites. By extending the lengths of these constructs, we found that several different Rap1p site arrays could never be found at the very distal end of a telomere, even when correctly oriented. Instead, these synthetic arrays were always followed by a short ( approximately 100-bp) "cap" of genuine TG repeat sequence, indicating a remarkably strict sequence requirement for an end-specific function(s) of the telomere. Despite this fact, even misoriented Rap1p site arrays promote telomere formation when they are placed at the distal end of a telomere-healing substrate, provided that at least a single correctly oriented site is present within the array. Surprisingly, these heterogeneous arrays of Rap1p binding sites generate telomeres through a RAD52-dependent fusion resolution reaction that results in an inversion of the original array. Our results provide new insights into the nature of telomere end capping and reveal one way by which recombination can resolve a defect in this process.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.