ArticleMolecular and cellular biology2001
Site-specific DNA binding of the Schizosaccharomyces pombe origin recognition complex is determined by the Orc4 subunit.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 98 citations in OpenAlex.
- Stranded short nascent strand sequencing reveals the topology of DNA replication origins ineLife · 2026Article
- The origin recognition complex requires chromatin tethering by a hypervariable intrinsically disordered region that is functionally conserved from sponge to man.Nucleic acids research · 2024Article
- The Origin Recognition Complex: From Origin Selection to Replication Licensing in Yeast and Humans.Biology · 2023Review
- Deletion of Orc4 during oogenesis severely reduces polar body extrusion and blocks zygotic DNA replication†.Biology of reproduction · 2022Article
- A composite DNA element that functions as a maintainer required for epigenetic inheritance of heterochromatin.Molecular cell · 2021Article
- Bioinformatical dissection of fission yeast DNA replication origins.Open biology · 2020Article
- Dynamics of DNA replication in a eukaryotic cell.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- From structure to mechanism-understanding initiation of DNA replication.Genes & development · 2017Review
- Sap1 is a replication-initiation factor essential for the assembly of pre-replicative complex in the fission yeastThe Journal of biological chemistry · 2017Article
- Mechanisms and regulation of DNA replication initiation in eukaryotes.Critical reviews in biochemistry and molecular biology · 2017Review
- Specific binding of eukaryotic ORC to DNA replication origins depends on highly conserved basic residues.Scientific reports · 2015Article
- Orc1 Binding to Mitotic Chromosomes Precedes Spatial Patterning during G1 Phase and Assembly of the Origin Recognition Complex in Human Cells.The Journal of biological chemistry · 2015Article
- DNA-protein interaction dynamics at the Lamin B2 replication origin.Cell cycle (Georgetown, Tex.) · 2015Article
- R-loops and initiation of DNA replication in human cells: a missing link?Frontiers in genetics · 2015Article
- Regulating DNA replication in eukarya.Cold Spring Harbor perspectives in biology · 2013Review
- Activation of a human chromosomal replication origin by protein tethering.Nucleic acids research · 2013Article
- Helicase loading at chromosomal origins of replication.Cold Spring Harbor perspectives in biology · 2013Review
- Chromatin architectures at fission yeast transcriptional promoters and replication origins.Nucleic acids research · 2012Article
- OriDB, the DNA replication origin database updated and extended.Nucleic acids research · 2012Article
- Identification of two telomere-proximal fission yeast DNA replication origins constrained by nearby cis-acting sequences to replicate in late S phase.F1000Research · 2012Article
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2 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The mechanism by which origin recognition complexes (ORCs) identify replication origins was investigated using purified Orc proteins from Schizosaccharomyces pombe. Orc4p alone bound tightly and specifically to several sites within S. pombe replication origins that are genetically required for origin activity. These sites consisted of clusters of A or T residues on one strand but were devoid of either alternating A and T residues or GC-rich sequences. Addition of a complex consisting of Orc1, -2, -3, -5, and -6 proteins (ORC-5) altered neither Orc4p binding to origin DNA nor Orc4p protection of specific sequences. ORC-5 alone bound weakly and nonspecifically to DNA; strong binding required the presence of Orc4p. Under these conditions, all six subunits remained bound to chromatin isolated from each phase of the cell division cycle. These results reveal that the S. pombe ORC binds to multiple, specific sites within replication origins and that site selection, at least in vitro, is determined solely by the Orc4p subunit.
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