ArticleMolecular and cellular biology2001
Highly frequent frameshift DNA synthesis by human DNA polymerase mu.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 84 citations in OpenAlex.
- For the Better or for the Worse? The Effect of Manganese on the Activity of Eukaryotic DNA Polymerases.International journal of molecular sciences · 2023Review
- Structural and Molecular Kinetic Features of Activities of DNA Polymerases.International journal of molecular sciences · 2022Review
- AP endonuclease 1 prevents the extension of a T/G mismatch by DNA polymerase β to prevent mutations in CpGs during base excision repair.DNA repair · 2016Article
- Structures of the Leishmania infantum polymerase beta.DNA repair · 2014Article
- Polymerase theta-mediated end joining of replication-associated DNA breaks in C. elegans.Genome research · 2014Article
- Molecular basis for DNA double-strand break annealing and primer extension by an NHEJ DNA polymerase.Cell reports · 2013Article
- Biological and therapeutic relevance of nonreplicative DNA polymerases to cancer.Antioxidants & redox signaling · 2013Review
- DNA expansions generated by human Polμ on iterative sequences.Nucleic acids research · 2013Article
- Yeast pol4 promotes tel1-regulated chromosomal translocations.PLoS genetics · 2013Article
- "Gate-keeper" residues and active-site rearrangements in DNA polymerase μ help discriminate non-cognate nucleotides.PLoS computational biology · 2013Article
- Translesion DNA synthesis in the context of cancer research.Cancer cell international · 2011Article
- Polymerases in nonhomologous end joining: building a bridge over broken chromosomes.Antioxidants & redox signaling · 2011Review
- Modeling DNA polymerase μ motions: subtle transitions before chemistry.Biophysical journal · 2010Article
- Error-prone DNA repair activity during somatic hypermutation in shark B lymphocytes.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- DNA pol λ's extraordinary ability to stabilize misaligned DNA.Journal of the American Chemical Society · 2010Article
- DNA polymerase family X: function, structure, and cellular roles.Biochimica et biophysica acta · 2010Review
- Conferring a template-dependent polymerase activity to terminal deoxynucleotidyltransferase by mutations in the Loop1 region.Nucleic acids research · 2009Article
- Characterization of DNA polymerase X from Thermus thermophilus HB8 reveals the POLXc and PHP domains are both required for 3'-5' exonuclease activity.Nucleic acids research · 2009Article
- A role for DNA polymerase mu in the emerging DJH rearrangements of the postgastrulation mouse embryo.Molecular and cellular biology · 2009Article
- The X family portrait: structural insights into biological functions of X family polymerases.DNA repair · 2007Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
DNA polymerase mu (Polmu) is a newly identified member of the polymerase X family. The biological function of Polmu is not known, although it has been speculated that human Polmu may be a somatic hypermutation polymerase. To help understand the in vivo function of human Polmu, we have performed in vitro biochemical analyses of the purified polymerase. Unlike any other DNA polymerases studied thus far, human Polmu catalyzed frameshift DNA synthesis with an unprecedentedly high frequency. In the sequence contexts examined, -1 deletion occurred as the predominant DNA synthesis mechanism opposite the single-nucleotide repeat sequences AA, GG, TT, and CC in the template. Thus, the fidelity of DNA synthesis by human Polmu was largely dictated by the sequence context. Human Polmu was able to efficiently extend mismatched bases mainly by a frameshift synthesis mechanism. With the primer ends, containing up to four mismatches, examined, human Polmu effectively realigned the primer to achieve annealing with a microhomology region in the template several nucleotides downstream. As a result, human Polmu promoted microhomology search and microhomology pairing between the primer and the template strands of DNA. These results show that human Polmu is much more prone to cause frameshift mutations than base substitutions. The biochemical properties of human Polmu suggest a function in nonhomologous end joining and V(D)J recombination through its microhomology searching and pairing activities but do not support a function in somatic hypermutation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.