ArticleMolecular and cellular biology2001
The Hsp70-Ydj1 molecular chaperone represses the activity of the heme activator protein Hap1 in the absence of heme.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 51 citations in OpenAlex.
- Transcriptional regulation of ergosterol biosynthesis genes in response to iron deficiency.Environmental microbiology · 2022Article
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- The Loz1 transcription factor from Schizosaccharomyces pombe binds to Loz1 response elements and represses gene expression when zinc is in excess.Molecular microbiology · 2019Article
- Heme promotes transcriptional and demethylase activities of Gis1, a member of the histone demethylase JMJD2/KDM4 family.Nucleic acids research · 2018Article
- Tetrapyrrole-based drought stress signalling.Plant biotechnology journal · 2015Review
- Reconsidering the nature and mode of action of metabolite retrograde signals from the chloroplast.Frontiers in plant science · 2012Article
- Kluyveromyces lactis: a suitable yeast model to study cellular defense mechanisms against hypoxia-induced oxidative stress.Oxidative medicine and cellular longevity · 2012Review
- Review
- Hsp90 regulates NADPH oxidase activity and is necessary for superoxide but not hydrogen peroxide production.Antioxidants & redox signaling · 2011Article
- The role of PAS kinase in PASsing the glucose signal.Sensors (Basel, Switzerland) · 2010Review
- Regulation and function of yeast PAS kinase: a role in the maintenance of cellular integrity.Cell cycle (Georgetown, Tex.) · 2009Article
- Farnesylation of Ydj1 is required for in vivo interaction with Hsp90 client proteins.Molecular biology of the cell · 2008Article
- Heme levels switch the function of Hap1 of Saccharomyces cerevisiae between transcriptional activator and transcriptional repressor.Molecular and cellular biology · 2007Article
- Regulation of the HAP1 gene involves positive actions of histone deacetylases.Biochemical and biophysical research communications · 2007Article
- Physiological evaluation of the filamentous fungus Trichoderma reesei in production processes by marker gene expression analysis.BMC biotechnology · 2007Article
- Nuclear export of the transcription factor NirA is a regulatory checkpoint for nitrate induction in Aspergillus nidulans.Molecular and cellular biology · 2007Article
- Characterization and prediction of protein-protein interactions within and between complexes.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Transcriptional monitoring of steady state and effects of anaerobic phases in chemostat cultures of the filamentous fungus Trichoderma reesei.BMC genomics · 2006Article
- The heme activator protein Hap1 represses transcription by a heme-independent mechanism in Saccharomyces cerevisiae.Genetics · 2005Article
- Structural environment dictates the biological significance of heme-responsive motifs and the role of Hsp90 in the activation of the heme activator protein Hap1.Molecular and cellular biology · 2003Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
In Saccharomyces cerevisiae, heme directly mediates the effects of oxygen on transcription through the heme activator protein Hap1. In the absence of heme, Hap1 is bound by at least four cellular proteins, including Hsp90 and Ydj1, forming a higher-order complex, termed HMC, and its activity is repressed. Here we purified the HMC and showed by mass spectrometry that two previously unidentified major components of the HMC are the Ssa-type Hsp70 molecular chaperone and Sro9 proteins. In vivo functional analysis, combined with biochemical analysis, strongly suggests that Ssa proteins are critical for Hap1 repression in the absence of heme. Ssa may repress the activities of both Hap1 DNA-binding and activation domains. The Ssa cochaperones Ydj1 and Sro9 appear to assist Ssa in Hap1 repression, and only Ydj1 residues 1 to 172 containing the J domain are required for Hap1 repression. Our results suggest that Ssa-Ydj1 and Sro9 act together to mediate Hap1 repression in the absence of heme and that molecular chaperones promote heme regulation of Hap1 by a mechanism distinct from the mechanism of steroid signaling.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.