ArticleMolecular and cellular biology2001
Methylation-mediated proviral silencing is associated with MeCP2 recruitment and localized histone H3 deacetylation.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed, 98 citations in OpenAlex.
- Precision prebiotics: Engineering food-derived polysaccharides to target specific SCFA-producing taxa for neuroprotection via the microbiota-gut-brain axis.Current research in food science · 2026Review
- Shaking up the silence: consequences of HMGN1 antagonizing PRC2 in the Down syndrome brain.Epigenetics & chromatin · 2022Review
- The Molecular Functions of MeCP2 in Rett Syndrome Pathology.Frontiers in genetics · 2021Review
- MKRN3 regulates the epigenetic switch of mammalian puberty via ubiquitination of MBD3.National science review · 2020Article
- Article
- Stem Cells Engineered During Different Stages of Reprogramming Reveal Varying Therapeutic Efficacies.Stem cells (Dayton, Ohio) · 2018Article
- Kinetics and epigenetics of retroviral silencing in mouse embryonic stem cells defined by deletion of the D4Z4 element.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Fine mapping of Xq28: both MECP2 and IRAK1 contribute to risk for systemic lupus erythematosus in multiple ancestral groups.Annals of the rheumatic diseases · 2013Article
- Transcriptional provirus silencing as a crosstalk of de novo DNA methylation and epigenomic features at the integration site.Nucleic acids research · 2012Article
- Epileptogenesis: can the science of epigenetics give us answers?Epilepsy currents · 2012Article
- Epigenetic regulation of transcription and splicing of syncytins, fusogenic glycoproteins of retroviral origin.Nucleic acids research · 2011Article
- H3K9me3-binding proteins are dispensable for SETDB1/H3K9me3-dependent retroviral silencing.Epigenetics & chromatin · 2011Article
- Constitutive heterochromatin reorganization during somatic cell reprogramming.The EMBO journal · 2011Article
- Lysine methyltransferase G9a is required for de novo DNA methylation and the establishment, but not the maintenance, of proviral silencing.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Cell line-dependent variability in HIV activation employing DNMT inhibitors.Virology journal · 2010Article
- Article
- Article
- MECP2 isoform-specific vectors with regulated expression for Rett syndrome gene therapy.PloS one · 2009Article
- Preferential epigenetic suppression of the autonomous MusD over the nonautonomous ETn mouse retrotransposons.Molecular and cellular biology · 2009Article
- Specific in vivo expression in type II pneumocytes of the Jaagsiekte sheep retrovirus long terminal repeat in transgenic mice.Virology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
The majority of 5-methylcytosine in mammalian DNA resides in endogenous transposable elements and is associated with the transcriptional silencing of these parasitic elements. Methylation also plays an important role in the silencing of exogenous retroviruses. One of the difficulties inherent in the study of proviral silencing is that the sites in which proviruses randomly integrate influence the probability of de novo methylation and expression. In order to compare methylated and unmethylated proviruses at the same genomic site, we used a recombinase-based targeting approach to introduce an in vitro methylated or unmethylated Moloney murine leukemia-based provirus in MEL cells. The methylated and unmethylated states are maintained in vivo, with the exception of the initially methylated proviral enhancer, which becomes demethylated in vivo. Although the enhancer is unmethylated and remodeled, the methylated provirus is transcriptionally silent. To further analyze the repressed state, histone acetylation status was determined by chromatin immunoprecipitation (ChIP) analyses, which revealed that localized histone H3 but not histone H4 hyperacetylation is inversely correlated with proviral methylation density. Since members of the methyl-CpG binding domain (MBD) family of proteins recruit histone deacetylase activity, these proteins may play a role in proviral repression. Interestingly, only MBD3 and MeCP2 are expressed in MEL cells. ChIPs with antibodies specific for these proteins revealed that only MeCP2 associates with the provirus in a methylation-dependent manner. Taken together, our results suggest that MeCP2 recruitment to a methylated provirus is sufficient for transcriptional silencing, despite the presence of a remodeled enhancer.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.