ArticleMolecular and cellular biology2001
Repression of Ets-2-induced transactivation of the tau interferon promoter by Oct-4.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 100 citations in OpenAlex.
- Pluripotent Core in Bovine Embryos: A Review.Animals : an open access journal from MDPI · 2022Review
- Integration of molecules to construct the processes of conceptus implantation to the maternal endometrium.Journal of animal science · 2018Review
- Transcriptional control ofReproduction (Cambridge, England) · 2017Review
- Master regulators in development: Views from the Drosophila retinal determination and mammalian pluripotency gene networks.Developmental biology · 2017Review
- A mRNA landscape of bovine embryos after standard and MAPK-inhibited culture conditions: a comparative analysis.BMC genomics · 2015Article
- Presence of Transcription Factor OCT4 Limits Interferon-tau Expression during the Pre-attachment Period in Sheep.Asian-Australasian journal of animal sciences · 2013Article
- Transcriptional regulation of two conceptus interferon tau genes expressed in Japanese black cattle during peri-implantation period.PloS one · 2013Article
- Generating pluripotent stem cells: differential epigenetic changes during cellular reprogramming.FEBS letters · 2012Review
- Optimization of a lipitoid-based plasmid DNA transfection protocol for bovine trophectoderm CT-1 cells.In vitro cellular & developmental biology. Animal · 2012Article
- Functions of interferon tau as an immunological regulator for establishment of pregnancy.Reproductive medicine and biology · 2012Review
- Squelching of ETS2 transactivation by POU5F1 silences the human chorionic gonadotropin CGA subunit gene in human choriocarcinoma and embryonic stem cells.Molecular endocrinology (Baltimore, Md.) · 2012Article
- Expression and quantification of Oct-4 gene in blastocyst and embryonic stem cells derived from in vitro produced buffalo embryos.In vitro cellular & developmental biology. Animal · 2012Article
- TF-Cluster: a pipeline for identifying functionally coordinated transcription factors via network decomposition of the shared coexpression connectivity matrix (SCCM).BMC systems biology · 2011Article
- Expression of bovine interferon-tau variants according to sex and age of conceptuses.Theriogenology · 2009Article
- Interferons and the maternal-conceptus dialog in mammals.Seminars in cell & developmental biology · 2008Review
- Combinatorial roles of protein kinase A, Ets2, and 3',5'-cyclic-adenosine monophosphate response element-binding protein-binding protein/p300 in the transcriptional control of interferon-tau expression in a trophoblast cell line.Molecular endocrinology (Baltimore, Md.) · 2008Article
- The EWS-Oct-4 fusion gene encodes a transforming gene.The Biochemical journal · 2007Article
- Ets-2 and C/EBP-beta are important mediators of ovine trophoblast Kunitz domain protein-1 gene expression in trophoblast.BMC molecular biology · 2007Article
- Plasticity of epidermal stem cells: survival in various environments.Stem cell reviews · 2005Review
- Trophoblast differentiation during embryo implantation and formation of the maternal-fetal interface.The Journal of clinical investigation · 2004Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Oct-4 is a POU family transcription factor associated with potentially totipotent cells. Genes expressed in the trophectoderm but not in embryos prior to blastocyst formation may be targets for silencing by Oct-4. Here, we have tested this hypothesis with the tau interferon genes (IFNT genes), which are expressed exclusively in the trophectoderm of bovine embryos. IFNT promoters contain an Ets-2 enhancer, located at -79 to -70, and are up-regulated about 20-fold by the overexpression of Ets-2 in human JAr choriocarcinoma cells, which are permissive for IFNT expression. This enhancement was reversed in a dose-dependent manner by coexpression of Oct-4 but not either Oct-1 or Oct-2. When cells were transfected with truncated bovine IFNT promoters designed to eliminate potential octamer sites sequentially, luciferase reporter expression from each construct was still silenced by Oct-4. Full repression required both the N-terminal and POU domains of Oct-4, but neither domain used alone was an effective silencer. Oct-4 and Ets-2 formed a complex in vitro in the absence of DNA through binding of the POU domain of Oct-4 to a site located between the "pointed" and DNA binding domains of Ets-2. The two transcription factors were also coimmunoprecipitated after being expressed together in JAr cells. Oct-4, therefore, silences IFNT promoters by quenching Ets-2 transactivation. The POU domain most probably binds to Ets-2 directly, while the N-terminal domain inhibits transcription. These findings provide further evidence that the developmental switch to the trophectoderm is accompanied by the loss of Oct-4 silencing of key genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.