ArticleJournal of virology2001
A single amino acid in the reverse transcriptase domain of hepatitis B virus affects virus replication efficiency.
Article in Journal of virology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 59 citations in OpenAlex.
- miR-HCC2 suppresses hepatitis B virus replication by inhibiting the activity of the enhancer I/X promoter.Archives of virology · 2023Article
- Spacer Domain in Hepatitis B Virus Polymerase: Plugging a Hole or Performing a Role?Journal of virology · 2022Review
- Hepatitis B Virus Small Envelope Protein Promotes Hepatocellular Carcinoma Angiogenesis via Endoplasmic Reticulum Stress Signaling To Upregulate the Expression of Vascular Endothelial Growth Factor A.Journal of virology · 2022Article
- Distinctive HBV Replication Capacity and Susceptibility to Tenofovir Induced by a Polymerase Point Mutation in Hepatoma Cell Lines and Primary Human Hepatocytes.International journal of molecular sciences · 2021Article
- Paradoxical HBsAg and anti-HBs coexistence among Chronic HBV Infections: Causes and Consequences.International journal of biological sciences · 2021Review
- Article
- Discovery and Selection of Hepatitis B Virus-Derived T Cell Epitopes for Global Immunotherapy Based on Viral Indispensability, Conservation, and HLA-Binding Strength.Journal of virology · 2020Article
- Hepatitis B Virus Surface Antigen Enhances the Sensitivity of Hepatocytes to Fas-Mediated Apoptosis via Suppression of AKT Phosphorylation.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Quantifying perinatal transmission of Hepatitis B viral quasispecies by tag linkage deep sequencing.Scientific reports · 2017Article
- Hepatitis B Virus-Encoded MicroRNA Controls Viral Replication.Journal of virology · 2017Article
- Modeling the functional state of the reverse transcriptase of hepatitis B virus and its application to probing drug-protein interaction.BMC bioinformatics · 2016Article
- Support of the infectivity of hepatitis delta virus particles by the envelope proteins of different genotypes of hepatitis B virus.Journal of virology · 2014Article
- Envelope proteins derived from naturally integrated hepatitis B virus DNA support assembly and release of infectious hepatitis delta virus particles.Journal of virology · 2014Article
- Novel natural mutations in the hepatitis B virus reverse transcriptase domain associated with hepatocellular carcinoma.PloS one · 2014Article
- Substitution rtq267h of hepatitis B virus increases the weight of replication and Lamivudine resistance.Hepatitis monthly · 2013Article
- Novel evidence suggests Hepatitis B virus surface proteins participate in regulation of HBV genome replication.Virologica Sinica · 2011Article
- Discovery and development of anti-HBV agents and their resistance.Molecules (Basel, Switzerland) · 2010Review
- Genomic variability associated with the presence of occult hepatitis B virus in HIV co-infected individuals.Journal of viral hepatitis · 2010Article
- In vitro drug susceptibility analysis of hepatitis B virus clinical quasispecies populations.Journal of clinical microbiology · 2007Article
- Emergence of a novel lamivudine-resistant hepatitis B virus variant with a substitution outside the YMDD motif.Antimicrobial agents and chemotherapy · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To explore functional domains in the hepatitis B virus (HBV) polymerase, two naturally occurring HBV isolates (56 and 2-18) with 98.7% nucleic acid sequence homology but different replication efficiencies were studied. After transfection into HepG2 cells, HBV DNA isolated from intracellular virus core particles was much higher in 56-transfected cells than in cells transfected with 2-18. The structural basis for the difference in replication efficiency between these two isolates was studied by functional domain gene substitution. The complete polymerase (P) gene and its gene segments coding for the terminal protein (TP), spacer (SP), reverse transcriptase (RT), and RNase H in 2-18 were separately replaced with their counterparts from 56 to construct full-length chimeric genomes. Cell transfection analysis revealed that substitution of the complete P gene of 2-18 with the P gene from 56 slightly enhanced viral replication. The only chimeric genome that regained the high replication efficiency of the original 56 isolate was the one with substitution of the RT gene of 2-18 with that from 56. Within the RT region, amino acid differences between isolates 2-18 and 56 were located at positions 617 (methionine versus leucine), 652 (serine versus proline), and 682 (valine versus leucine). Point mutation identified amino acid 652 as being responsible for the difference in replication efficiency. Homologous modeling studies of the HBV RT domain suggest that the mutation of residue 652 from proline to serine might affect the conformation of HBV RT which interacts with the template-primer, leading to impaired polymerase activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.