ArticleClinical and diagnostic laboratory immunology2001
Early events in macrophage killing of Aspergillus fumigatus conidia: new flow cytometric viability assay.
Article in Clinical and diagnostic laboratory immunology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 50 citations in OpenAlex.
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- Pathogenicity and virulence ofVirulence · 2023Review
- Extracellular vesicles derived fromFrontiers in immunology · 2023Article
- Computational Modeling of Macrophage Iron Sequestration during Host Defense against Aspergillus.mSphere · 2022Article
- The Glycosylphosphatidylinositol-Anchored Superoxide Dismutase ofJournal of fungi (Basel, Switzerland) · 2021Article
- Phenomic profiling of a novel sibling species within the Scedosporium complex in Thailand.BMC microbiology · 2021Article
- Deficiency of GPI Glycan Modification by Ethanolamine Phosphate Results in Increased Adhesion and Immune Resistance ofFrontiers in cellular and infection microbiology · 2021Article
- Pseudomonas aeruginosa Inhibits the Growth of Scedosporium and Lomentospora In Vitro.Mycopathologia · 2018Article
- Flow Cytometry Is a Powerful Tool for Assessment of the Viability of Fungal Conidia in Metalworking Fluids.Applied and environmental microbiology · 2017Article
- Tissue-Resident Macrophages in Fungal Infections.Frontiers in immunology · 2017Review
- Interaction of THP-1 Monocytes with Conidia and Hyphae of DifferentFrontiers in immunology · 2017Article
- Corticosteroids block autophagy protein recruitment in Aspergillus fumigatus phagosomes via targeting dectin-1/Syk kinase signaling.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Impaired bactericidal but not fungicidal activity of polymorphonuclear neutrophils in patients with chronic lymphocytic leukemia.Leukemia & lymphoma · 2013Article
- The immune interplay between the host and the pathogen in Aspergillus fumigatus lung infection.BioMed research international · 2013Review
- Germination of Aspergillus fumigatus inside avian respiratory macrophages is associated with cytotoxicity.Veterinary research · 2012Article
- Cystic fibrosis transmembrane conductance regulator regulates epithelial cell response to Aspergillus and resultant pulmonary inflammation.American journal of respiratory and critical care medicine · 2012Article
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- Fluorescent viability stains to probe the metabolic status of aflatoxigenic fungus in dual culture of Aspergillus flavus and Pichia anomala.Mycopathologia · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Detailed investigations of macrophage phagocytosis and killing of Aspergillus fumigatus conidia have been limited by technical difficulties in quantifying fungal uptake and viability. In order to study early events in cell pathogen ingestion and killing, we developed a new flow cytometry assay that utilizes the fungus-specific viability dye FUN-1. Metabolically active A. fumigatus conidia accumulate orange fluorescence in vacuoles, while dormant or dead conidia stain green. After incubation within THP-1 cells, recovered conidia are costained with propidium iodide (PI) to discriminate between dormant and dead cells. Flow cytometric measurements of FUN-1 metabolism and PI uptake provide indicators of conidial viability, dormancy, and death. Conidial phagocytosis and killing are also assessed by measurement of green and orange FUN-1 fluorescence within the THP-1 cell population. Compared to previously described methods, this assay has less error introduced by membrane permeability changes and serial dilution of filamentous fungal forms. Results suggest that the THP-1 cells kill conidia rapidly (within 6 h) after exposure. Conidia that are preexposed to human serum are ingested and killed more quickly than are nonopsonized conidia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.