ArticleClinical and diagnostic laboratory immunology2001
Mycobacterial di-O-acyl-trehalose inhibits mitogen- and antigen-induced proliferation of murine T cells in vitro.
Article in Clinical and diagnostic laboratory immunology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- Linking lipid profile alterations to antibiotic tolerance and natural product synergy in drug-resistant Mycobacterium tuberculosis clinical isolates.Scientific reports · 2026Article
- Pathogenicity and virulence ofVirulence · 2023Review
- Spotlight on mycobacterial lipid exploitation using nanotechnology for diagnosis, vaccines, and treatments.Nanomedicine : nanotechnology, biology, and medicine · 2023Review
- Immunological hyporesponsiveness in tuberculosis: The role of mycobacterial glycolipids.Frontiers in immunology · 2022Review
- Chemical Synthesis of Cell Wall Constituents ofChemical reviews · 2021Review
- Complete Characterization of Polyacyltrehaloses fromBiochemistry · 2021Article
- Asymmetric Total Synthesis of Mycobacterial Diacyl Trehaloses Demonstrates a Role for Lipid Structure in Immunogenicity.ACS chemical biology · 2020Article
- The thick waxy coat of mycobacteria, a protective layer against antibiotics and the host's immune system.The Biochemical journal · 2020Review
- Formation of Lung Inducible Bronchus Associated Lymphoid Tissue Is Regulated byFrontiers in immunology · 2020Article
- Underestimated Manipulative Roles ofFrontiers in immunology · 2019Review
- Simple glycolipids of microbes: Chemistry, biological activity and metabolic engineering.Synthetic and systems biotechnology · 2018Review
- Mycobacterial glycolipid Di-O-acyl trehalose promotes a tolerogenic profile in dendritic cells.PloS one · 2018Article
- Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences · 2018Review
- Comparative metabolic profiling of mce1 operon mutant vs wild-type Mycobacterium tuberculosis strains.Pathogens and disease · 2015Article
- Diversity of Mycobacterium tuberculosis across Evolutionary Scales.PLoS pathogens · 2015Article
- The rv1184c locus encodes Chp2, an acyltransferase in Mycobacterium tuberculosis polyacyltrehalose lipid biosynthesis.Journal of bacteriology · 2015Article
- Differential response of porcine immature monocyte-derived dendritic cells to virulent and inactivated transmissible gastroenteritis virus.Research in veterinary science · 2014Article
- Biosynthesis and translocation of unsulfated acyltrehaloses in Mycobacterium tuberculosis.The Journal of biological chemistry · 2014Article
- A chloroplast-derived C4V3 polypeptide from the human immunodeficiency virus (HIV) is orally immunogenic in mice.Plant molecular biology · 2012Article
- A STAT4-dependent Th1 response is required for resistance to the helminth parasite Taenia crassiceps.Infection and immunity · 2004Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
2,3-Di-O-acyl-trehalose (DAT) is a glycolipid located on the outer layer of the Mycobacterium tuberculosis cell envelope. Due to its noncovalent linkage to the mycobacterial peptidoglycan, DAT could easily interact with host cells located in the focus of infection. The aim of the present work was to study the effects of DAT on the proliferation of murine spleen cells. DAT was purified from reference strains of M. tuberculosis, or M. fortuitum as a surrogate source of the compound, by various chromatography and solvent extraction procedures and then chemically identified. Incubation of mouse spleen cells with DAT inhibited in a dose-dependent manner concanavalin A-stimulated proliferation of the cells. Experiments, including the propidium iodide exclusion test, showed that these effects were not due to death of the cells. Tracking of cell division by labeling with 5,6-carboxyfluorescein diacetate succinimidyl ester revealed that DAT reduces the rounds of cell division. Immunofluorescence with an anti-CD3 monoclonal antibody indicated that T lymphocytes were the population affected in our model. Our experiments also suggest that the extent of the suppressive activity is strongly dependent on the structural composition of the acyl moieties in DATs. Finally, the inhibitory effect was also observed on antigen-induced proliferation of mouse spleen cells specific for Toxoplasma gondii. All of these data suggest that DAT could have a role in the T-cell hyporesponsiveness observed in chronic tuberculosis.
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