ArticleDevelopment (Cambridge, England)2001
spiel ohne grenzen/pou2 is required during establishment of the zebrafish midbrain-hindbrain boundary organizer.
Article in Development (Cambridge, England), 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 114 citations in OpenAlex.
- Genetically encoded affinity reagents are a toolkit for visualizing and manipulating endogenous protein function in vivo.Nature communications · 2025Article
- Genetic Mechanism That Defines the Characteristic Neurogenesis Patterns in the Neural Plate Using Hes/her Genes During Early Vertebrate Development.Genesis (New York, N.Y. : 2000) · 2025Article
- Transcriptome Analysis Suggested Striking Transition Around the End of Epiboly in the Gene Regulatory Network Downstream of the Oct4-Type POU Gene in Zebrafish Embryos.Development, growth & differentiation · 2025Article
- Kick-starting the zygotic genome: licensors, specifiers, and beyond.EMBO reports · 2024Review
- A simple and scalable zebrafish model of Sonic hedgehog medulloblastoma.Cell reports · 2024Article
- Development, circuitry, and function of the zebrafish cerebellum.Cellular and molecular life sciences : CMLS · 2023Review
- Evolutionary origin of vertebrate OCT4/POU5 functions in supporting pluripotency.Nature communications · 2022Article
- Identification of embryonic RNA granules that act as sites of mRNA translation after changing their physical properties.iScience · 2022Article
- The functional diversity of the POUV-class proteins across vertebrates.Open biology · 2022Review
- Pluripotency factors determine gene expression repertoire at zygotic genome activation.Nature communications · 2022Article
- An Update on the Molecular Mechanism of the Vertebrate Isthmic Organizer Development in the Context of the Neuromeric Model.Frontiers in neuroanatomy · 2022Review
- Identification ofGenetics · 2018Article
- Zebrafish Znfl1 proteins control the expression ofThe Journal of biological chemistry · 2017Article
- Evolutionary Conservation of pou5f3 Genomic Organization and Its Dynamic Distribution during Embryogenesis and in Adult Gonads in Japanese Flounder Paralichthys olivaceus.International journal of molecular sciences · 2017Article
- Article
- Role of miRNA-9 in Brain Development.Journal of experimental neuroscience · 2016Review
- Gene structure and identification of minimal promoter of Pou2 expressed in spermatogonial cells of rohu carp, Labeo rohita.Molecular biology reports · 2014Article
- Pou5f1/Oct4 promotes cell survival via direct activation of mych expression during zebrafish gastrulation.PloS one · 2014Article
- Novel mechanisms that pattern and shape the midbrain-hindbrain boundary.Cellular and molecular life sciences : CMLS · 2013Review
- Oct transcription factors in development and stem cells: insights and mechanisms.Development (Cambridge, England) · 2013Review
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 3 countries.
Funding
Abstract
The vertebrate midbrain-hindbrain boundary (MHB) organizes patterning and neuronal differentiation in the midbrain and anterior hindbrain. Formation of this organizing center involves multiple steps, including positioning of the MHB within the neural plate, establishment of the organizer and maintenance of its regional identity and signaling activities. Juxtaposition of the Otx2 and Gbx2 expression domains positions the MHB. How the positional information is translated into activation of Pax2, Wnt1 and Fgf8 expression during MHB establishment remains unclear. In zebrafish spiel ohne grenzen (spg) mutants, the MHB is not established, neither isthmus nor cerebellum form, the midbrain is reduced in size and patterning abnormalities develop within the hindbrain. In spg mutants, despite apparently normal expression of otx2, gbx1 and fgf8 during late gastrula stages, the initial expression of pax2.1, wnt1 and eng2, as well as later expression of fgf8 in the MHB primordium are reduced. We show that spg mutants have lesions in pou2, which encodes a POU-domain transcription factor. Maternal pou2 transcripts are distributed evenly in the blastula, and zygotic expression domains include the midbrain and hindbrain primordia during late gastrulation. Microinjection of pou2 mRNA can rescue pax2.1 and wnt1 expression in the MHB of spg/pou2 mutants without inducing ectopic expression. This indicates an essential but permissive role for pou2 during MHB establishment. pou2 is expressed normally in noi/pax2.1 and ace/fgf8 zebrafish mutants, which also form no MHB. Thus, expression of pou2 does not depend on fgf8 and pax2.1. Our data suggest that pou2 is required for the establishment of the normal expression domains of wnt1 and pax2.1 in the MHB primordium.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.