ArticleJournal of clinical microbiology2001
Differentiation of Candida albicans and Candida dubliniensis by fluorescent in situ hybridization with peptide nucleic acid probes.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 94 citations in OpenAlex.
- Artificial genetic polymers against human pathologies.Biology direct · 2022Review
- Identification ofJournal of fungi (Basel, Switzerland) · 2021Article
- Application of Culture-Independent Rapid Diagnostic Tests in the Management of Invasive Candidiasis and Cryptococcosis.Journal of fungi (Basel, Switzerland) · 2015Review
- Community structure of gut fungi during different developmental stages of the Chinese white pine beetle (Dendroctonus armandi).Scientific reports · 2015Article
- Design and evaluation of peptide nucleic acid probes for specific identification of Candida albicans.Journal of clinical microbiology · 2015Article
- Molecular diagnosis of sepsis: New aspects and recent developments.European journal of microbiology & immunology · 2014Article
- Development of a peptide nucleic acid probe to Trichosporon species and identification of trichosporonosis by use of in situ hybridization in formalin-fixed and paraffin-embedded (FFPE) sections.Journal of clinical microbiology · 2013Article
- Detection and identification of microorganisms in wine: a review of molecular techniques.Journal of industrial microbiology & biotechnology · 2011Review
- Identification of Fusarium species in formalin-fixed and paraffin-embedded sections by in situ hybridization using peptide nucleic acid probes.Journal of clinical microbiology · 2011Article
- Efflux-mediated antifungal drug resistance.Clinical microbiology reviews · 2009Review
- Peptide nucleic acid fluorescent in situ hybridization for hospital-acquired enterococcal bacteremia: delivering earlier effective antimicrobial therapy.Antimicrobial agents and chemotherapy · 2008Article
- DNA mimics for the rapid identification of microorganisms by fluorescence in situ hybridization (FISH).International journal of molecular sciences · 2008Article
- [Molecular biological detection of pathogens in patients with sepsis. Potentials, limitations and perspectives].Der Anaesthesist · 2008Review
- Rapid and accurate identification of Candida albicans isolates by use of PNA FISHFlow.Journal of clinical microbiology · 2008Article
- Multicenter evaluation of the Candida albicans/Candida glabrata peptide nucleic acid fluorescent in situ hybridization method for simultaneous dual-color identification of C. albicans and C. glabrata directly from blood culture bottles.Journal of clinical microbiology · 2008Article
- Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species.Journal of clinical microbiology · 2007Article
- Peptide nucleic acid fluorescence in situ hybridization-based identification of Candida albicans and its impact on mortality and antifungal therapy costs.Journal of clinical microbiology · 2006Article
- Faster identification of pathogens in positive blood cultures by fluorescence in situ hybridization in routine practice.Journal of clinical microbiology · 2006Article
- Multicenter evaluation of a Candida albicans peptide nucleic acid fluorescent in situ hybridization probe for characterization of yeast isolates from blood cultures.Journal of clinical microbiology · 2005Article
- In situ accessibility of Saccharomyces cerevisiae 26S rRNA to Cy3-labeled oligonucleotide probes comprising the D1 and D2 domains.Applied and environmental microbiology · 2003Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The recent discovery of Candida dubliniensis as a separate species that traditionally has been identified as Candida albicans has led to the development of a variety of biochemical and molecular methods for the differentiation of these two pathogenic yeasts. rRNA sequences are well-established phylogenetic markers, and probes targeting species-specific rRNA sequences have been used in diagnostic assays for the detection and identification of microorganisms. Peptide nucleic acid (PNA) is a DNA mimic with improved hybridization characteristics, and the neutral backbone of PNA probes offers significant advantages in whole-cell in situ hybridization assays. In this study, we developed PNA probes targeting the rRNAs of C. albicans and C. dubliniensis and applied them to a fluorescence in situ hybridization method (PNA FISH) for differentiation between C. albicans and C. dubliniensis. Liquid cultures were smeared onto microscope slides, heat fixed, and then hybridized for 30 min. Unhybridized PNA probe was removed by washing, and smears were examined by fluorescence microscopy. Evaluation of the PNA FISH method using smears of 79 C. dubliniensis and 70 C. albicans strains showed 100% sensitivity and 100% specificity for both PNA probes. We concluded that PNA FISH is a powerful tool for the differentiation of C. albicans and C. dubliniensis.
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