ArticleJournal of clinical microbiology2001
Diagnostic examination of human intestinal spirochetosis by fluorescent in situ hybridization for Brachyspira aalborgi, Brachyspira pilosicoli, and other species of the genus Brachyspira (Serpulina).
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 2 of them syntheses that pooled it.
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Who cites it
20 citing papers in PubMed, 2 syntheses or guidelines pooled it, 78 citations in OpenAlex.
- Clinical and Pathologic Factors Associated With Colonic Spirochete (Brachyspira pilosicoli and Brachyspira aalborgi) Infection: A Comprehensive Systematic Review and Pooled Analysis.American journal of clinical pathology · 2023Pooled it
- Human intestinal spirochetosis, irritable bowel syndrome, and colonic polyps: A systematic review and meta-analysis.Journal of gastroenterology and hepatology · 2022Pooled it
- Human Intestinal Spirochetosis Shows Higher Positivity in Sessile Serrated Lesions Than in Tubular Adenomas With Preferential Colonization of Absorptive Epithelium.Pathology international · 2026Article
- Food-chain spirochetes: a unified hypothesis for Parkinson's disease and dementia risk.Frontiers in aging neuroscience · 2026Article
- Human Intestinal Spirochetosis as a Cause of Chronic Diarrhea in an Immunocompetent Patient: A Case Report.Cureus · 2026Article
- Identification of Terminal βGlcNAc onThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2024Article
- Article
- The comparison of the intensity of human intestinal spirochetes betweenJournal of clinical biochemistry and nutrition · 2019Article
- Treponema pallidum Immunohistochemistry is positive in human intestinal Spirochetosis.Diagnostic pathology · 2018Article
- The Spirochete Brachyspira pilosicoli, Enteric Pathogen of Animals and Humans.Clinical microbiology reviews · 2018Review
- Appendiceal spirochaetosis in children.Gut pathogens · 2013Article
- Development of a real-time PCR for identification of brachyspira species in human colonic biopsies.PloS one · 2012Article
- Lactobacilli antagonize the growth, motility, and adherence of Brachyspira pilosicoli: a potential intervention against avian intestinal spirochetosis.Applied and environmental microbiology · 2011Article
- Human intestinal spirochetosis--a review.German medical science : GMS e-journal · 2010Review
- Rapid and accurate diagnosis of human intestinal spirochetosis by fluorescence in situ hybridization.Journal of clinical microbiology · 2009Article
- Spatial organisation of microbiota in quiescent adenoiditis and tonsillitis.Journal of clinical pathology · 2007Article
- Comparison of prevalence and risk factors for faecal carriage of the intestinal spirochaetes Brachyspira aalborgi and Brachyspira pilosicoli in four Australian populations.Epidemiology and infection · 2006Article
- Spatial organization and composition of the mucosal flora in patients with inflammatory bowel disease.Journal of clinical microbiology · 2005Article
- Spatial organization of bacterial flora in normal and inflamed intestine: a fluorescence in situ hybridization study in mice.World journal of gastroenterology · 2005Article
- Detection by PCR and isolation assays of the anaerobic intestinal spirochete Brachyspira aalborgi from the feces of captive nonhuman primates.Journal of clinical microbiology · 2003Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human intestinal spirochetosis, characterized by end-on attachment of densely packed spirochetes to the epithelial surface of the large intestines as a fringe has been associated with the weakly beta-hemolytic spirochetes Brachyspira aalborgi and Brachyspira (Serpulina) pilosicoli. In this study, fluorescent in situ hybridization with oligonucleotide probes targeting 16S or 23S rRNA of B. aalborgi, B. pilosicoli, and the genus Brachyspira was applied to 40 sections of formalin-fixed, paraffin-embedded intestinal biopsy specimens from 23 Danish and 15 Norwegian patients with histologic evidence of intestinal spirochetosis. Five biopsy specimens from patients without intestinal spirochetosis and three samples from pigs with experimental B. pilosicoli colitis were examined as well. In addition, the 16S ribosomal DNAs of two clinical isolates of B. aalborgi were sequenced, and a PCR procedure was developed for the identification of B. aalborgi in cultures. The genotypic characteristics of the two clinical isolates showed very high (99.5%) similarity with two existing isolates, the type strain of B. aalborgi and a Swedish isolate. Hybridization with the Brachyspira genus-specific probe revealed a brightly fluorescing fringe of spirochetes on the epithelia of 39 biopsy specimens, whereas 1 biopsy specimen was hybridization negative. The spirochetes in biopsy specimens from 13 Danish and 8 Norwegian patients (55.3%) were identified as B. aalborgi. The spirochetes in the biopsy specimens from the other 17 patients hybridized only with the Brachyspira probe, possibly demonstrating the involvement of as-yet-uncharacterized Brachyspira spirochetes in human intestinal spirochetosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.