ArticleJournal of clinical microbiology2001
Combined PCR-heteroduplex mobility assay for detection and differentiation of influenza A viruses from different animal species.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish.Scientific reports · 2020Article
- Development of Nested PCR-Heteroduplex Mobility Assay for Determination of Genetic Diversity in the Block 2 Region of theJournal of parasitology research · 2020Article
- An optimized molecular method for detection of influenza A virus using improved generic primers and concentration of the viral genomic RNA and nucleoprotein complex.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2019Article
- European surveillance of emerging pathogens associated with canine infectious respiratory disease.Veterinary microbiology · 2017Article
- Experimental infection of dogs with H3N2 canine influenza virus from China.Epidemiology and infection · 2013Article
- Influenza A virus infection of healthy piglets in an abattoir in Brazil: animal-human interface and risk for interspecies transmission.Memorias do Instituto Oswaldo Cruz · 2013Article
- Genetic drift evolution under vaccination pressure among H5N1 Egyptian isolates.Virology journal · 2011Article
- Strain typing of Mycoplasma cynos isolates from dogs with respiratory disease.Veterinary microbiology · 2009Article
- Rapid differentiation of influenza A virus subtypes and genetic screening for virus variants by high-resolution melting analysis.Journal of clinical microbiology · 2008Article
- Avian influenza: genetic evolution under vaccination pressure.Virology journal · 2008Article
- Influenza and respiratory syncytial virus infections in British Hajj pilgrims.Emerging health threats journal · 2008Article
- Design and validation of an H5 TaqMan real-time one-step reverse transcription-PCR and confirmatory assays for diagnosis and verification of influenza A virus H5 infections in humans.Journal of clinical microbiology · 2007Article
- Genetic variability and molecular epidemiology of respiratory syncytial virus subgroup a strains in Japan determined by heteroduplex mobility assay.Journal of clinical microbiology · 2004Article
- Influenza AH1N2 viruses, United Kingdom, 2001-02 influenza season.Emerging infectious diseases · 2003Article
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2 authors.
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Abstract
Transfer of influenza A viruses from animal hosts to man may lead to the emergence of new human pandemic strains. The early detection and identification of such events are therefore paramount in the surveillance of influenza viruses. To detect and partially characterize influenza A viruses from different animal species, a combined reverse transcription (RT)-PCR heteroduplex mobility assay (HMA) was designed. This M gene RT-PCR was shown to be sensitive and specific for the detection of human, avian, and swine influenza A viruses. PCR amplicons from human, avian, and swine viruses of 15 different subtypes, with between 1.9 and 21.4% nucleotide divergence, were differentiated by HMA. Sequencing of the amplicons showed that the heteroduplex mobility patterns correlated with the sequence divergence between test and reference DNA. The application of the RT-PCR HMA method for rapid screening of samples was assessed with a reference panel of viruses of human, avian, and swine origin. The avian H9N2 virus A/HongKong/1073/99, which crossed the species barrier to humans, was screened against the reference panel. It was found to be most closely related to the avian A/Quail/HongKong/G1/97 H9N2 reference PCR product. Sequence analysis showed a nucleotide divergence of 1.1% between the A/Quail/HongKong/G1/97 and A/HongKong/1073/99 amplicons. From the results of our work, we consider the RT-PCR HMA method described to offer a rapid and sensitive means for screening for novel or unusual influenza viruses.
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