ArticleJournal of clinical microbiology2001
Analysis of microsatellite markers of Candida albicans used for rapid typing.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 121 citations in OpenAlex.
- Short Tandem Repeat Genotyping of Medically Important Fungi: A Comprehensive Review of a Powerful Tool with Extensive Future Potential.Mycopathologia · 2024Review
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- The Gastrointestinal Tract Is Pinpointed as a Reservoir ofJournal of fungi (Basel, Switzerland) · 2023Article
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- Genotyping Reveals High Clonal Diversity and Widespread Genotypes ofFrontiers in cellular and infection microbiology · 2020Article
- Reduction in Percentage of Clusters of Candida albicans and Candida parapsilosis Causing Candidemia in a General Hospital in Madrid, Spain.Journal of clinical microbiology · 2018Article
- Microsatellite genotyping of clinical Candida parapsilosis isolates.Current medical mycology · 2017Article
- The intraspecies diversity of C. albicans triggers qualitatively and temporally distinct host responses that determine the balance between commensalism and pathogenicity.Mucosal immunology · 2017Article
- Investigating Clinical Issues by Genotyping of Medically Important Fungi: Why and How?Clinical microbiology reviews · 2017Review
- Echinocandin Resistance in Candida Species Isolates from Liver Transplant Recipients.Antimicrobial agents and chemotherapy · 2017Article
- Post-transcriptional regulation of transcript abundance by a conserved member of the tristetraprolin family in Candida albicans.Molecular microbiology · 2015Article
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- Candida identification: a journey from conventional to molecular methods in medical mycology.World journal of microbiology & biotechnology · 2014Review
- Review
- Endemic genotypes of Candida albicans causing fungemia are frequent in the hospital.Journal of clinical microbiology · 2013Article
- Analysis of strain relatedness using high resolution melting in a case of recurrent candiduria.BMC microbiology · 2013Article
- Molecular fingerprints to identify Candida species.BioMed research international · 2013Review
- Candida glabrata strain relatedness by new microsatellite markers.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Article
- Multilocus microsatellite markers for molecular typing of Candida glabrata: application to analysis of genetic relationships between bloodstream and digestive system isolates.Journal of clinical microbiology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To obtain a rapid genotyping method of Candida albicans, three polymorphic microsatellite markers were investigated by multiplex PCR. The three loci, called CDC3, EF3, and HIS3, were chosen because they are on different chromosomes so as to improve the chances of finding polymorphisms. One set of primers was designed for each locus, and one primer of each set was dye-labeled to read PCR signals by using an automatic sequencer. Amplifications were performed directly from the colonies harvested on the agar plate without a sophisticated DNA extraction step. At total of 27 reference strains and 73 clinical independent isolates were tested. The numbers of allelic associations were 10, 22, and 25 for the loci CDC3, EF3, and HIS3, respectively. The combined discriminatory power of the three microsatellites markers was 0.97. The markers were stable after 25 subcultures, and the amplifications were specific for C. albicans. An initial study of 17 clinical isolate pairs, including blood culture and peripheral sites, showed a similar genotype for 15 of them, confirming that candidemia usually originates from the colonizing isolate. Therefore, microsatellite marker analysis with multiplex PCR and automated procedures has a high throughput and should be suitable for large epidemiologic studies of C. albicans.
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