ArticleJournal of clinical microbiology2001
Burden of unidentifiable mycobacteria in a reference laboratory.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 88 citations in OpenAlex.
- Evolution of Laboratory Diagnosis of Tuberculosis.Clinics and practice · 2024Review
- Mycobacteriosis and Tuberculosis: Laboratory Diagnosis.The open microbiology journal · 2018Review
- The rebirth of culture in microbiology through the example of culturomics to study human gut microbiota.Clinical microbiology reviews · 2015Article
- Role of GenoType(®) Mycobacterium Common Mycobacteria/Additional Species Assay for Rapid Differentiation Between Mycobacterium tuberculosis Complex and Different Species of Non-Tuberculous Mycobacteria.Journal of laboratory physicians · 2013Article
- Tuberculosis diagnostics: Challenges and opportunities.Lung India : official organ of Indian Chest Society · 2012Article
- Chronic pulmonary disease due to Mycobacterium monacense infection: the first case from Iran.Annals of laboratory medicine · 2012Article
- The Use of High Performance Liquid Chromatography to Speciate and Characterize the Epidemiology of Mycobacteria.Laboratory medicine · 2011Article
- Multi-probe real-time PCR identification of common Mycobacterium species in blood culture broth.The Journal of molecular diagnostics : JMD · 2009Article
- Mycobacterium alsiense, a novel, slowly growing species isolated from two patients with pulmonary disease.Journal of clinical microbiology · 2007Article
- Mycobacterium avium in the postgenomic era.Clinical microbiology reviews · 2007Review
- Evaluation of the GenoType Mycobacterium Assay for identification of mycobacterial species from cultures.Journal of clinical microbiology · 2006Article
- Description of Mycobacterium conceptionense sp. nov., a Mycobacterium fortuitum group organism isolated from a posttraumatic osteitis inflammation.Journal of clinical microbiology · 2006Article
- Sequence-based identification of new bacteria: a proposition for creation of an orphan bacterium repository.Journal of clinical microbiology · 2005Review
- Infections due to the newly described species Mycobacterium parascrofulaceum.Journal of clinical microbiology · 2005Article
- Detection and genotyping of Mycobacterium species from clinical isolates and specimens by oligonucleotide array.Journal of clinical microbiology · 2005Article
- Identification of Mycobacterium species by secA1 sequences.Journal of clinical microbiology · 2005Article
- Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases.Clinical microbiology reviews · 2004Review
- Systematic 16S rRNA gene sequencing of atypical clinical isolates identified 27 new bacterial species associated with humans.Journal of clinical microbiology · 2004Article
- Health impacts of environmental mycobacteria.Clinical microbiology reviews · 2004Review
- rpoB-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteria.Journal of clinical microbiology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 9 institutions in 7 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Modern identification techniques at the genomic level have greatly improved the taxonomic knowledge of mycobacteria. In adjunct to nucleic acid sequences, mycobacterial identification has been endorsed by investigation of the lipidic patterns of unique mycolic acids in such organisms. In the present investigation, the routine use of high-performance liquid chromatography (HPLC) of mycolic acids, followed by the sequencing of the 16S rRNA, allowed us to select 72 mycobacterial strains, out of 1,035 screened, that do not belong to any of the officially recognized mycobacterial species. Most strains (i.e., 47) were isolated from humans, 13 were from the environment, 3 were from animals, and 9 were from unknown sources. The majority of human isolates were grown from the respiratory tract and were therefore most likely not clinically significant. Some, however, were isolated from sterile sites (blood, pleural biopsy, central venous catheter, or pus). Many isolates, including several clusters of two or more strains, mostly slow growers and scotochromogenic, presented unique genetic and lipidic features. We hope the data reported here, including the results of major conventional identification tests, the HPLC profiles of strains isolated several times, and the whole sequences of the 16S rRNA hypervariable regions of all 72 mycobacteria, may encourage reporting of new cases. The taxonomy of the genus Mycobacterium is, in our opinion, still far from being fully elucidated, and the reporting of unusual strains provides the best background for the recognition of new species. Our report also shows the usefulness of the integration of novel technology to routine diagnosis, especially in cases involving slow-growing microorganisms such as mycobacteria.
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