ArticleJournal of clinical microbiology2001
Polymorphic internal transcribed spacer region 1 DNA sequences identify medically important yeasts.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
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Who cites it
80 citing papers in PubMed, 239 citations in OpenAlex.
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- Isolation and identification of Candida tropicalis in sows with fatal infection: a case report.BMC veterinary research · 2021Article
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- Multilocus sequence typing of Candida albicans isolates from the oral cavities of patients undergoing haemodialysis.Scientific reports · 2018Article
- Microbial Identification Using DNA Target Amplification and Sequencing: Clinical Utility and Impact on Patient Management.Open forum infectious diseases · 2018Review
- Case Report: Histoplasmosis: First Autochthonous Case from Israel.The American journal of tropical medicine and hygiene · 2018Article
- Methods of Candida dubliniensis identification and its occurrence in human clinical material.Folia microbiologica · 2017Article
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20 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Species-specific polymorphisms in the noncoding internal transcribed spacer 2 (ITS2) region of the rRNA operon provide accurate identification of clinically significant yeasts. In this study, we tested the hypothesis that ITS1 noncoding regions contain diagnostically useful alleles. The length of ITS1 region PCR products amplified from 40 species (106 clinical strains, 5 reference strains, and 30 type strains) was rapidly determined with single-base precision by automated capillary electrophoresis. Polymorphisms in the PCR product length permitted 19 species to be distinguished by ITS1 alone, compared with 16 species distinguished by using only ITS2. However, combination of both ITS alleles permitted identification of 30 species (98% of clinical isolates). The remaining 10 species with PCR products of similar sizes contained unique ITS alleles distinguishable by restriction enzyme analysis. DNA sequence analysis of amplified ITS1 region DNA from 79 isolates revealed species-specific ITS1 alleles for each of the 40 pathogenic species examined. This provided identification of unusual clinical isolates, and 53 diagnostic ITS1 sequences were deposited in GenBank. Phylogenetic analyses based on ITS sequences showed a similar overall topology to 26S rRNA gene-based trees. However, different species with identical 26S sequences contained distinct ITS alleles that provided species identification with strong statistical support. Together, these data indicate that the analysis of ITS polymorphisms can reliably identify 40 species of clinically significant yeasts and that the capacity for identifying potentially new pathogenic species by using this database holds significant promise.
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