ArticleJournal of clinical microbiology2001
Alternative identification test relying upon sexual reproductive abilities of Candida lusitaniae strains isolated from hospitalized patients.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Clinical Characteristics and Mortality Risk Factors ofAnnals of laboratory medicine · 2026Article
- Fpr1p mediates the synergistic effect of rapamycin or tacrolimus with caspofungin inJAC-antimicrobial resistance · 2026Article
- Mediator Subunit Med15 Regulates Cell Morphology and Mating inJournal of fungi (Basel, Switzerland) · 2023Article
- Review
- Using Genomics to Shape the Definition of the Agglutinin-Like Sequence (Frontiers in cellular and infection microbiology · 2021Article
- Candida lusitaniae in Kuwait: Prevalence, antifungal susceptibility and role in neonatal fungemia.PloS one · 2019Article
- Sexual reproduction of human fungal pathogens.Cold Spring Harbor perspectives in medicine · 2014Review
- Deletion of the uracil permease gene confers cross-resistance to 5-fluorouracil and azoles in Candida lusitaniae and highlights antagonistic interaction between fluorinated nucleotides and fluconazole.Antimicrobial agents and chemotherapy · 2014Article
- Article
- The deacetylase Sir2 from the yeast Clavispora lusitaniae lacks the evolutionarily conserved capacity to generate subtelomeric heterochromatin.PLoS genetics · 2013Article
- Nonsense and missense mutations in FCY2 and FCY1 genes are responsible for flucytosine resistance and flucytosine-fluconazole cross-resistance in clinical isolates of Candida lusitaniae.Antimicrobial agents and chemotherapy · 2009Article
- Mechanistic plasticity of sexual reproduction and meiosis in the Candida pathogenic species complex.Current biology : CB · 2009Article
- Insight into the role of HOG pathway components Ssk2p, Pbs2p, and Hog1p in the opportunistic yeast Candida lusitaniae.Eukaryotic cell · 2008Article
- Contributions of the response regulators Ssk1p and Skn7p in the pseudohyphal development, stress adaptation, and drug sensitivity of the opportunistic yeast Candida lusitaniae.Eukaryotic cell · 2008Article
- Differential involvement of histidine kinase receptors in pseudohyphal development, stress adaptation, and drug sensitivity of the opportunistic yeast Candida lusitaniae.Eukaryotic cell · 2007Article
- Inactivation of the FCY2 gene encoding purine-cytosine permease promotes cross-resistance to flucytosine and fluconazole in Candida lusitaniae.Antimicrobial agents and chemotherapy · 2005Article
- Differentiation between atypical isolates of Candida lusitaniae and Candida pulcherrima by determination of mating type.Journal of clinical microbiology · 2005Article
- Disruption of ergosterol biosynthesis confers resistance to amphotericin B in Candida lusitaniae.Antimicrobial agents and chemotherapy · 2003Article
- Flucytosine-fluconazole cross-resistance in purine-cytosine permease-deficient Candida lusitaniae clinical isolates: indirect evidence of a fluconazole uptake transporter.Antimicrobial agents and chemotherapy · 2003Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The in vitro mating ability of Candida lusitaniae (teleomorph Clavispora lusitaniae) clinical isolates has been investigated. Studying the effects of culture conditions, we showed that ammonium ion depletion in the medium is a major trigger of the sexual cycle. Moreover, a solid support is required for mating, suggesting a role for adhesion factors in addition to the mating type gene recognition function. Monitoring of mating and meiosis efficiency with auxotrophic strains showed great variations in ascospore yields, which appeared to be strain and temperature dependent, with an optimal range of 18 to 28 degrees C. The morphogenetic events taking place from mating to ascospore release were studied by scanning and electron microscopy, and the ultrastructure of the conjugation canal, through which intercellular nuclear exchanges occur, was revealed. Labeling experiments with a lectin-fluorochrome system revealed that the nuclear transfer was predominantly polarized, thus allowing a distinction between the nucleus donor and the nucleus acceptor strains. The direction of the transfer depended on the strain combination used, rather than on the genotypes of the strains, and did not appear to be controlled by the mating type genes. Finally, we demonstrated that all of the 76 clinical isolates used in this study were able to reproduce sexually when mated with an opposite mating type strain, and we identified a 1:1 MATa/MATalpha ratio in the collection. These results support the idea that there is no anamorph state in C. lusitaniae. Accordingly, the mating type test, which is easy to use and can usually be completed within 48 h, is a reliable alternative identification system for C. lusitaniae.
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