ArticleRNA (New York, N.Y.)2001
A mutant viral RNA promoter with an altered conformation retains efficient recognition by a viral RNA replicase through a solution-exposed adenine.
Article in RNA (New York, N.Y.), 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- Subgenomic promoter recognition by the norovirus RNA-dependent RNA polymerases.Nucleic acids research · 2015Article
- Review
- RNA binding by the brome mosaic virus capsid protein and the regulation of viral RNA accumulation.Journal of molecular biology · 2009Article
- Brome mosaic virus capsid protein regulates accumulation of viral replication proteins by binding to the replicase assembly RNA element.RNA (New York, N.Y.) · 2009Article
- RNA recognition and cleavage by the SARS coronavirus endoribonuclease.Journal of molecular biology · 2006Article
- Interaction between Brome mosaic virus proteins and RNAs: effects on RNA replication, protein expression, and RNA stability.Journal of virology · 2005Article
- Repair of the tRNA-like CCA sequence in a multipartite positive-strand RNA virus.Journal of virology · 2005Article
- NMR structures of double loops of an RNA aptamer against mammalian initiation factor 4A.Nucleic acids research · 2005Article
- Requirements for brome mosaic virus subgenomic RNA synthesis in vivo and replicase-core promoter interactions in vitro.Journal of virology · 2004Article
- Template sequence near the initiation nucleotide can modulate brome mosaic virus RNA accumulation in plant protoplasts.Journal of virology · 2004Article
- Brome mosaic virus RNA syntheses in vitro and in barley protoplasts.Journal of virology · 2003Article
- Stable RNA structures can repress RNA synthesis in vitro by the brome mosaic virus replicase.RNA (New York, N.Y.) · 2003Article
- Enhancer-like activity of a brome mosaic virus RNA promoter.Journal of virology · 2003Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brome mosaic virus (BMV) genomic minus-strand RNA synthesis requires an RNA motif named stem-loop C (SLC). An NMR-derived solution structure of SLC was reported by Kim et al. (Nature Struc Biol, 2000, 7:415-423) to contain three replicase-recognition elements, the most important of which is a stable stem with a terminal trinucleotide loop, 5'AUA3'. The 5'-most adenine of the triloop is rigidly fixed to the stem helix by interactions that require the 3'-most adenine, which is called a clamped adenine motif. However, a change of the 3' adenine to guanine (5'AUG3') unexpectedly directed RNA synthesis at 130% of wild type (Kim et al., Nature Struc Biol, 2000, 7:415-423). To understand how RNA with the AUG mutation maintains interaction with the BMV replicase, we used NMR and other biophysical techniques to elucidate the solution conformation of a 13-nt RNA containing the AUG triloop, called S-AUG. We found that S-AUG has a drastically different loop conformation in comparison to the wild type, as evidenced by an unusual C x G loop-closing base pair. Despite the conformational change, S-AUG maintains a solution-exposed adenine similar to the clamped adenine motif found in the wild type. Biochemical studies of the 5'AUG3' loop with various substitutions in the context of the whole SLC construct confirm that the clamped adenine motif exists in S-AUG remains a primary structural feature required for RNA synthesis by the BMV replicase.
Indexed as
Identifiers
11680852PMC1370191W2121313543What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.