ArticleActa crystallographica. Section D, Biological crystallography2001
Streptococcus pneumonia YlxR at 1.35 A shows a putative new fold.
Article in Acta crystallographica. Section D, Biological crystallography, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 24 citations in OpenAlex.
- The previously uncharacterized RnpM (YlxR) protein modulates the activity of ribonuclease P in Bacillus subtilis in vitro.Nucleic acids research · 2024Article
- Interactors and effects of overexpressing YlxR/RnpM, a conserved RNA binding protein in cyanobacteria.RNA biology · 2024Article
- A model industrial workhorse: Bacillus subtilis strain 168 and its genome after a quarter of a century.Microbial biotechnology · 2023Review
- Grad-seq identifies KhpB as a global RNA-binding protein inmicroLife · 2021Article
- Article
- Newly Identified Nucleoid-Associated-Like Protein YlxR Regulates Metabolic Gene Expression in Bacillus subtilis.mSphere · 2018Article
- Defining a minimal cell: essentiality of small ORFs and ncRNAs in a genome-reduced bacterium.Molecular systems biology · 2015Article
- Inspecting the potential physiological and biomedical value of 44 conserved uncharacterised proteins of Streptococcus pneumoniae.BMC genomics · 2014Article
- Structure-based functional inference of hypothetical proteins from Mycoplasma hyopneumoniae.Journal of molecular modeling · 2012Article
- YbxF and YlxQ are bacterial homologs of L7Ae and bind K-turns but not K-loops.RNA (New York, N.Y.) · 2012Article
- Phylogenomics of prokaryotic ribosomal proteins.PloS one · 2012Article
- Structural basis for ubiquitin recognition by a novel domain from human phospholipase A2-activating protein.The Journal of biological chemistry · 2009Article
- The structural role of the zinc ion can be dispensable in prokaryotic zinc-finger domains.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Novel algorithms reveal streptococcal transcriptomes and clues about undefined genes.PLoS computational biology · 2007Article
- Automation of protein purification for structural genomics.Journal of structural and functional genomics · 2004Article
- Structural classification of zinc fingers: survey and summary.Nucleic acids research · 2003Article
- JEvTrace: refinement and variations of the evolutionary trace in JAVA.Genome biology · 2002Article
- Review
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The structure of the YlxR protein of unknown function from Streptococcus pneumonia was determined to 1.35 A. YlxR is expressed from the nusA/infB operon in bacteria and belongs to a small protein family (COG2740) that shares a conserved sequence motif GRGA(Y/W). The family shows no significant amino-acid sequence similarity with other proteins. Three-wavelength diffraction MAD data were collected to 1.7 A from orthorhombic crystals using synchrotron radiation and the structure was determined using a semi-automated approach. The YlxR structure resembles a two-layer alpha/beta sandwich with the overall shape of a cylinder and shows no structural homology to proteins of known structure. Structural analysis revealed that the YlxR structure represents a new protein fold that belongs to the alpha-beta plait superfamily. The distribution of the electrostatic surface potential shows a large positively charged patch on one side of the protein, a feature often found in nucleic acid-binding proteins. Three sulfate ions bind to this positively charged surface. Analysis of potential binding sites uncovered several substantial clefts, with the largest spanning 3/4 of the protein. A similar distribution of binding sites and a large sharply bent cleft are observed in RNA-binding proteins that are unrelated in sequence and structure. It is proposed that YlxR is an RNA-binding protein.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.