Evidence map›Paper›PMID 11679362›Full record

ArticleApplied and environmental microbiology2001

Rapid extraction of DNA From Escherichia coli and Cryptosporidium parvum for use in PCR.

J A Higgins, M C Jenkins, D R Shelton, R Fayer, J S Karns

Open access · greenAbstract readEvaluation Study
In one paragraph

Article in Applied and environmental microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 30 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

J A HigginsU.S. Department of Agriculture-Agricultural Research Service, Beltsville, Maryland 20705, USA. jhiggins@anri.barc.usda.gov
M C Jenkins
D R Shelton
R Fayer
J S Karns
Agricultural Research Service · USUnited States Department of Agriculture · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The Xtra Amp tube, Isocode paper, Instagene matrix, and PrepMan matrix methods were evaluated for their ability to rapidly extract PCR-quality DNAs from Escherichia coli O157:H7 and Cryptosporidium parvum. All methods provided satisfactory DNA from E. coli, and the Xtra Amp and Instagene reagents provided satisfactory DNA from C. parvum.

Indexed as

AnimalsCryptosporidium parvumDNA, BacterialDNA, ProtozoanEscherichia coli O157Nucleic Acid Amplification TechniquesPolymerase Chain ReactionDNA, BacterialDNA, Protozoan

Identifiers

PMID11679362
PMCPMC93307
OpenAlexW2113143143

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.