ArticleApplied and environmental microbiology2001
Detection of the isiA gene across cyanobacterial strains: potential for probing iron deficiency.
Article in Applied and environmental microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 40 citations in OpenAlex.
- Coupling of excitation energy to photochemistry in natural marine phytoplankton communities under iron stress.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Function of the IsiA pigment-protein complex in vivo.Photosynthesis research · 2019Article
- Biogeography of CyanobacterialFrontiers in microbiology · 2019Article
- IsiA is required for the formation of photosystem I supercomplexes and for efficient state transition in synechocystis PCC 6803.PloS one · 2010Article
- Article
- Unraveling the genomic mosaic of a ubiquitous genus of marine cyanobacteria.Genome biology · 2008Article
- A pair of iron-responsive genes encoding protein kinases with a Ser/Thr kinase domain and a His kinase domain are regulated by NtcA in the Cyanobacterium Anabaena sp. strain PCC 7120.Journal of bacteriology · 2006Article
- Detection of Prochlorothrix in brackish waters by specific amplification of pcb genes.Applied and environmental microbiology · 2003Article
- Reflections on the function of IsiA, a cyanobacterial stress-inducible, Chl-binding protein.Photosynthesis researchArticle
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of isiA expression to monitor the iron status of cyanobacteria was investigated. Studies of laboratory cultures of the cyanobacterium Synechocystis sp. strain PCC 6803 showed that isiA expression is dependent on the organism's response to iron deficiency; isiA expression starts as soon as a decline in the rate of growth begins. isiA expression is switched on at concentrations of iron citrate of less than 0.7 microM. A PCR method was developed for the specific amplification of the iron-regulated isiA gene from a variety of cyanobacteria. After we developed degenerate primers, 15 new internal isiA fragments (840 bp) were amplified, cloned, and sequenced from strains obtained from algal collections, from new isolates, and from enriched field samples. Furthermore, isiA expression could be detected by means of reverse transcription-PCR when enriched field samples were exposed to restricted iron availability. These results imply that determining the level of iron-regulated isiA expression can serve to indicate iron deficiency in cyanobacterial samples of differing origins from the field.
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