ArticleApplied and environmental microbiology2001
Inhibition of Giardia intestinalis by extracellular factors from Lactobacilli: an in vitro study.
Article in Applied and environmental microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 115 citations in OpenAlex.
- Multifunctional antimicrobial effects ofFrontiers in immunology · 2026Article
- Detection and co-occurrence of Acanthamoeba and Klebsiella pneumoniae in freshwater river systems of Taichung, Taiwan.BMC microbiology · 2025Article
- Probing the functional and therapeutic properties of postbiotics in relation to their industrial application.Food science & nutrition · 2023Review
- The interaction of gut microbiota with parasitic protozoa.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- Probiotics in the management of Giardia duodenalis: an update on potential mechanisms and outcomes.Naunyn-Schmiedeberg's archives of pharmacology · 2021Review
- Bile Salt Hydrolases: At the Crossroads of Microbiota and Human Health.Microorganisms · 2021Review
- Parasite-Probiotic Interactions in the Gut:Frontiers in immunology · 2021Article
- Eukaryotic and Prokaryotic Microbiota Interactions.Microorganisms · 2020Review
- Parasite-bacteria interrelationship.Parasitology research · 2020Review
- Review
- In vitro and ex vivo evaluation of the anti-Giardia duodenalis activity of the supernatant of Slab51 (SivoMixx).PloS one · 2019Article
- The influence of probiotic bacteria and human gut microorganisms causing opportunistic infections onGut pathogens · 2019Article
- Recent insights into innate and adaptive immune responses to Giardia.Advances in parasitology · 2019Review
- The Anti-parasitic Effect of Probiotic BacteriaHelminthologia · 2018Article
- Parasite-Microbiota Interactions With the Vertebrate Gut: Synthesis Through an Ecological Lens.Frontiers in microbiology · 2018Review
- Bile Salt Hydrolase Activities: A Novel Target to Screen Anti-Frontiers in microbiology · 2018Article
- Micromanaging Immunity in the Murine Host vs. the Mosquito Vector: Microbiota-Dependent Immune Responses to Intestinal Parasites.Frontiers in cellular and infection microbiology · 2018Review
- Lactic acid bacteria as starter cultures: An update in their metabolism and genetics.AIMS microbiology · 2018Review
- The Intersection of Immune Responses, Microbiota, and Pathogenesis in Giardiasis.Trends in parasitology · 2017Review
- Parasitic Protozoa and Interactions with the Host Intestinal Microbiota.Infection and immunity · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of the present work was to evaluate the effect of spent culture supernatants of different strains of lactobacilli on giardia trophozoites. The growth of Giardia intestinalis strain WB, as well as the attachment to the human intestinal epithelial cell line Caco-2, was evaluated by using proliferation and adhesion assays with radiolabeled parasites. In addition, scanning electron microscopy and flow cytometric analysis were performed. The effect of spent culture supernatants from lactobacilli was strain dependent. Lactobacillus johnsonii La1 significantly inhibited the proliferation of G. intestinalis trophozoites. Although the effect was strongly pH dependent, it was not simply due to lactic acid. According to flow cytometric analysis, trophozoites were arrested in G(1) phase but neither significant necrosis nor apoptosis could be detected. Bacterial cells or their spent culture supernatants were unable to modify trophozoite attachment to Caco-2 cells. However, trophozoites treated with spent culture supernatants had little, if any, proliferative capacity. These results suggest that La1 produces some substance(s) able to inhibit proliferation of Giardia trophozoites. Partial characterization of the factors involved in the antigiardiasic action showed that they have a low molecular mass and are inactivated by heating. On this basis, it seems worthwhile to explore how colonization of the proximal small bowel with these lactic acid bacteria could interfere with giardiasis in vivo.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.