ArticleClinical and experimental immunology2001
Differentiation of antineutrophil nuclear antibodies in inflammatory bowel and autoimmune liver diseases from antineutrophil cytoplasmic antibodies (p-ANCA) using immunofluorescence microscopy.
Article in Clinical and experimental immunology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 68 citations in OpenAlex.
- Article
- Interference of anti-nuclear antibodies on determination of anti-neutrophil cytoplasmic antibodies in patients suspected of vasculitis: a case series.Biochemia medica · 2023Article
- Uncommon onset manifestations without renal involvement in microscopic polyangiitis: A case report.Experimental and therapeutic medicine · 2022Article
- Antineutrophil cytoplasmic antibody profiles differ according to type of primary sclerosing cholangitis and autoimmune hepatitis.Clinics (Sao Paulo, Brazil) · 2021Article
- Autoantibodies in Autoimmune Liver Disease-Clinical and Diagnostic Relevance.Frontiers in immunology · 2018Review
- Ectopic Tertiary Lymphoid Tissue in Inflammatory Bowel Disease: Protective or Provocateur?Frontiers in immunology · 2016Review
- Clinical Utility of Biomarkers in IBD.Current gastroenterology reports · 2015Review
- Autoimmune hepatitis in childhood: the role of genetic and immune factors.World journal of gastroenterology · 2013Review
- Automated interpretation of ANCA patterns - a new approach in the serology of ANCA-associated vasculitis.Arthritis research & therapy · 2012Article
- Autoimmune hepatitis as a unique form of an autoimmune liver disease: immunological aspects and clinical overview.Autoimmune diseases · 2012Article
- Contribution of immunofluorescence to identification and characterization of antineutrophil cytoplasmic antibodies in inflammatory bowel disease.Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology · 2011Article
- Evaluation of the combined application of ethanol-fixed and formaldehyde-fixed neutrophil substrates for identifying atypical perinuclear antineutrophil cytoplasmic antibodies in inflammatory bowel disease.Clinical and vaccine immunology : CVI · 2009Article
- Atypical p-ANCA in PSC and AIH: a hint toward a "leaky gut"?Clinical reviews in allergy & immunology · 2009Review
- Autoantibodies in primary sclerosing cholangitis.World journal of gastroenterology · 2008Review
- Autoimmune liver serology: current diagnostic and clinical challenges.World journal of gastroenterology · 2008Review
- Utility of serological markers in inflammatory bowel diseases: gadget or magic?World journal of gastroenterology · 2007Review
- Evolving diagnostic strategies for inflammatory bowel disease.Current gastroenterology reports · 2006Review
- Analysis of serum antibodies in patients suspected of having inflammatory bowel disease.Clinical and vaccine immunology : CVI · 2006Article
- Role of auto-antibodies for the diagnosis of chronic cholestatic liver diseases.Clinical reviews in allergy & immunology · 2005Review
- The effect of a symptom related "gating policy" on ANCA requests in routine clinical practice.Journal of clinical pathology · 2004Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Perinuclear antineutrophil cytoplasmic antibodies (p-ANCA) directed against cytoplasmic proteins of neutrophils have been studied extensively in patients with systemic vasculitides. Recent data indicate that antineutrophil antibodies in sera from patients with chronic inflammatory bowel diseases (IBD) or autoimmune liver disorders, currently called 'atypical p-ANCA', recognize a nuclear target antigen, rendering the term 'ANCA' inaccurate. Specific microscopic criteria to distinguish atypical p-ANCA from p-ANCA are lacking. We used planar and confocal laser scanning indirect immunofluorescence microscopy to examine the labelling characteristics of ethanol-, methanol- and formaldehyde-fixed neutrophils by antineutrophil antibodies in 153 serum samples from patients with IBD, autoimmune liver disorders, systemic vasculitides or healthy blood donors. On ethanol- or methanol-fixed neutrophils, multiple intranuclear fluorescent foci together with either a rim-like peripheral nuclear staining ('type A') or a combined cytoplasmic and peripheral nuclear staining ('type B') was noted exclusively with atypical p-ANCA in sera from patients with IBD or autoimmune liver disorders. Intranuclear foci, which probably corresponded to invaginations of the nuclear envelope, were not labelled by p-ANCA from patients with microscopic polyangiitis or cytoplasmic ANCA (c-ANCA) from patients with Wegener's granulomatosis. On formaldehyde-fixed neutrophils, atypical p-ANCA gave a fine rim-like staining of the nuclear periphery, whereas ANCA diffusely labelled the cytoplasm. To distinguish reliably between the patterns produced by atypical p-ANCA or p-ANCA, particularly p-ANCA, careful indirect immunofluorescence microscopy on ethanol- as well as on formaldehyde-fixed neutrophils is necessary, with particular emphasis on the presence of multiple intranuclear fluorescent foci.
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