ArticleThe Journal of cell biology2001
Transmission of growth cone traction force through apCAM-cytoskeletal linkages is regulated by Src family tyrosine kinase activity.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.
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Who cites it
60 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The Prognostic Significance of NEDD9 Expression in Human Cancers: A Systematic Review, Meta-Analysis, and Omics Exploration.Technology in cancer research & treatmentPooled it
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- A modified motor-clutch model reveals that neuronal growth cones respond faster to soft substrates.Molecular biology of the cell · 2024Article
- Measuring Retrograde Actin Flow in Neuronal Growth Cones.Methods in molecular biology (Clifton, N.J.) · 2024Article
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- Re-evaluating the actin-dependence of spectraplakin functions during axon growth and maintenance.Developmental neurobiology · 2022Article
- Signaling Enzymes and Ion Channels Being Modulated by the Actin Cytoskeleton at the Plasma Membrane.International journal of molecular sciences · 2021Review
- Unraveling Axon Guidance during Axotomy and Regeneration.International journal of molecular sciences · 2021Review
- Prion Protein at the Leading Edge: Its Role in Cell Motility.International journal of molecular sciences · 2020Review
- Axonal cytomechanics in neuronal development.Journal of biosciences · 2020Review
- A single tyrosine phosphorylation site in cortactin is important for filopodia formation in neuronal growth cones.Molecular biology of the cell · 2019Article
- Regulation of axon growth by myosin II-dependent mechanocatalysis of cofilin activity.The Journal of cell biology · 2019Article
- A low-cost microwell device for high-resolution imaging of neurite outgrowth in 3D.Journal of neural engineering · 2018Article
- An Integrated Cytoskeletal Model of Neurite Outgrowth.Frontiers in cellular neuroscience · 2018Review
- Using Single-Protein Tracking to Study Cell Migration.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Biochemical analysis of force-sensitive responses using a large-scale cell stretch device.Cell adhesion & migration · 2017Article
- Possible Reversal of PTSD-Related DNA Methylation by Sympathetic Blockade.Journal of molecular neuroscience : MN · 2017Review
- Local Arp2/3-dependent actin assembly modulates applied traction force during apCAM adhesion site maturation.Molecular biology of the cell · 2017Article
- Front-Rear Polarization by Mechanical Cues: From Single Cells to Tissues.Trends in cell biology · 2016Review
- Article
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Abstract
Tyrosine kinase activity is known to be important in neuronal growth cone guidance. However, underlying cellular mechanisms are largely unclear. Here, we report how Src family tyrosine kinase activity controls apCAM-mediated growth cone steering by regulating the transmission of traction forces through receptor-cytoskeletal linkages. Increased levels of tyrosine phosphorylation were detected at sites where beads coated with apCAM ligands were physically restrained to induce growth cone steering, but not at unrestrained bead binding sites. Interestingly, the rate and level of phosphotyrosine buildup near restrained beads were decreased by the myosin inhibitor 2,3-butanedione-2-monoxime, suggesting that tension promotes tyrosine kinase activation. While not affecting retrograde F-actin flow rates, genistein and the Src family selective tyrosine kinase inhibitors PP1 and PP2 strongly reduced the growth cone's ability to apply traction forces through apCAM-cytoskeletal linkages, assessed using the restrained bead interaction assay. Furthermore, increased levels of an activated Src family kinase were detected at restrained bead sites during growth cone steering events. Our results suggest a mechanism by which growth cones select pathways by sampling both the molecular nature of the substrate and its ability to withstand the application of traction forces.
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