ArticleJournal of bacteriology2001
The TOL plasmid pWW0 xylN gene product from Pseudomonas putida is involved in m-xylene uptake.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 79 citations in OpenAlex.
- Article
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- Generalization of Classification of AlkB Family Alkane Monooxygenases fromInternational journal of molecular sciences · 2025Article
- Genetic Bioaugmentation-Mediated Bioremediation of Terephthalate in Soil Microcosms Using an Engineered Environmental Plasmid.Microbial biotechnology · 2025Article
- Comparative Genomics of Rhamnolipid Synthesis and Monoaromatic Hydrocarbon Tolerance Genes in EnvironmentalF1000Research · 2024Article
- A Novel FadL Homolog, AltL, Mediates Transport of Long-Chain Alkanes and Fatty Acids in Acinetobacter venetianus RAG-1.Applied and environmental microbiology · 2022Article
- Uptake of monoaromatic hydrocarbons during biodegradation by FadL channel-mediated lateral diffusion.Nature communications · 2020Article
- Marine hydrocarbonoclastic bacteria as whole-cell biosensors for n-alkanes.Microbial biotechnology · 2015Article
- Degradation of benzene, toluene, and xylene isomers by a bacterial consortium obtained from rhizosphere soil of Cyperus sp. grown in a petroleum-contaminated area.Folia microbiologica · 2013Article
- Outer membrane protein AlkL boosts biocatalytic oxyfunctionalization of hydrophobic substrates in Escherichia coli.Applied and environmental microbiology · 2012Article
- The Crc global regulator inhibits the Pseudomonas putida pWW0 toluene/xylene assimilation pathway by repressing the translation of regulatory and structural genes.The Journal of biological chemistry · 2010Article
- A beta-barrel outer membrane protein facilitates cellular uptake of polychlorophenols in Cupriavidus necator.Biodegradation · 2010Article
- Broad-host-range expression vectors with tightly regulated promoters and their use to examine the influence of TraR and TraM expression on Ti plasmid quorum sensing.Applied and environmental microbiology · 2008Article
- Outer-membrane transport of aromatic hydrocarbons as a first step in biodegradation.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Bacterial metabolism of polycyclic aromatic hydrocarbons: strategies for bioremediation.Indian journal of microbiology · 2008Article
- Identification and characterization of genes encoding a putative ABC-type transporter essential for utilization of gamma-hexachlorocyclohexane in Sphingobium japonicum UT26.Journal of bacteriology · 2007Article
- The ColRS two-component system regulates membrane functions and protects Pseudomonas putida against phenol.Journal of bacteriology · 2006Article
- 4-Chlorobenzoate uptake in Comamonas sp. strain DJ-12 is mediated by a tripartite ATP-independent periplasmic transporter.Journal of bacteriology · 2006Article
- Cloning and functional characterization of the styE gene, involved in styrene transport in Pseudomonas putida CA-3.Applied and environmental microbiology · 2006Article
- Saturable, energy-dependent uptake of phenanthrene in aqueous phase by Mycobacterium sp. strain RJGII-135.Applied and environmental microbiology · 2004Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The upper operon of the TOL plasmid pWW0 of Pseudomonas putida encodes a set of enzymes involved in the conversion of toluene and xylenes to their carboxylic acid derivatives. The last gene of the upper operon, xylN, encodes a 465-amino-acid polypeptide which exhibits significant sequence similarity to FadL, an outer membrane protein involved in fatty acid transport in Escherichia coli. To analyze the role of the xylN gene product, xylN on TOL plasmid pWW0 was disrupted by inserting a kanamycin resistance gene, and the phenotypes of P. putida harboring the wild-type and xylN mutant TOL plasmids were characterized. The growth of P. putida harboring the wild-type TOL plasmid was inhibited by a high concentration of m-xylene, while that of P. putida harboring the xylN mutant TOL plasmid was not. The apparent K(s) value for the oxidation of m-xylene in intact cells of the xylN mutant was fourfold higher than that of the wild-type strain, although the TOL catabolic enzyme activities in cell extracts from the two strains were almost identical. We therefore presume that the xylN gene product is a porin involved in the transport of m-xylene and its analogues across the outer membrane. Western blot analysis confirmed the localization of XylN in the outer membrane.
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