ArticleJournal of bacteriology2001
Complementation of a nonmotile flaB mutant of Borrelia burgdorferi by chromosomal integration of a plasmid containing a wild-type flaB allele.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Genetic Manipulation ofCurrent issues in molecular biology · 2021Review
- Periplasmic flagella in Borrelia burgdoferi function to maintain cellular integrity upon external stress.PloS one · 2017Article
- Flagellar motility of the pathogenic spirochetes.Seminars in cell & developmental biology · 2015Review
- Article
- Motor rotation is essential for the formation of the periplasmic flagellar ribbon, cellular morphology, and Borrelia burgdorferi persistence within Ixodes scapularis tick and murine hosts.Infection and immunity · 2015Article
- Identification of three extra-chromosomal replicons in Leptospira pathogenic strain and development of new shuttle vectors.BMC genomics · 2015Article
- Use of an endogenous plasmid locus for stable in trans complementation in Borrelia burgdorferi.Applied and environmental microbiology · 2015Article
- Borrelia burgdorferi tissue morphologies and imaging methodologies.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2013Article
- Motility is crucial for the infectious life cycle of Borrelia burgdorferi.Infection and immunity · 2013Article
- Article
- The β₃-integrin ligand of Borrelia burgdorferi is critical for infection of mice but not ticks.Molecular microbiology · 2012Article
- The elastic basis for the shape of Borrelia burgdorferi.Biophysical journal · 2009Article
- The Borrelia burgdorferi flaB promoter has an extended -10 element and includes a T-rich -35/-10 spacer sequence that is essential for optimal activity.FEMS microbiology letters · 2009Article
- The flat-ribbon configuration of the periplasmic flagella of Borrelia burgdorferi and its relationship to motility and morphology.Journal of bacteriology · 2009Article
- Borrelia burgdorferi uniquely regulates its motility genes and has an intricate flagellar hook-basal body structure.Journal of bacteriology · 2008Article
- CheX is a phosphorylated CheY phosphatase essential for Borrelia burgdorferi chemotaxis.Journal of bacteriology · 2005Article
- Development of a novel chloramphenicol resistance expression plasmid used for genetic complementation of a fliG deletion mutant in Treponema denticola.Infection and immunity · 2004Article
- The decrease in FlaA observed in a flaB mutant of Borrelia burgdorferi occurs posttranscriptionally.Journal of bacteriology · 2004Article
- OspC facilitates Borrelia burgdorferi invasion of Ixodes scapularis salivary glands.The Journal of clinical investigation · 2004Article
- aadA confers streptomycin resistance in Borrelia burgdorferi.Journal of bacteriology · 2003Article
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Authors and funding
6 authors.
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Abstract
With the recent identification of antibiotic resistance phenotypes, the use of reporter genes, the isolation of null mutants by insertional inactivation, and the development of extrachromosomal cloning vectors, genetic analysis of Borrelia burgdorferi is becoming a reality. A previously described nonmotile, rod-shaped, kanamycin-resistant B. burgdorferi flaB::Km null mutant was complemented by electroporation with the erythromycin resistance plasmid pED3 (a pGK12 derivative) containing the wild-type flaB sequence and 366 bp upstream from its initiation codon. The resulting MS17 clone possessed erythromycin and kanamycin resistance, flat-wave morphology, and microscopic and macroscopic motility. Several other electroporations with plasmids containing wild-type flaB and various lengths (198, 366, or 762 bp) of sequence upstream from the flaB gene starting codon did not lead to functional restoration of the nonmotile flaB null mutant. DNA hybridization, PCR analysis, and sequencing indicated that the wild-type flaB gene in nonmotile clones was present in the introduced extrachromosomal plasmids, while the motile MS17 clone was a merodiploid containing single tandem chromosomal copies of mutated flaB::Km and wild-type flaB with a 366-bp sequence upstream from its starting codon. Complementation was thus achieved only when wild-type flaB was inserted into the borrelial chromosome. Several possible mechanisms for the failure of complementation for extrachromosomally located flaB are discussed.
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