ArticleThe British journal of ophthalmology2001
Detection of galectin-3 in tear fluid at disease states and immunohistochemical and lectin histochemical analysis in human corneal and conjunctival epithelium.
Article in The British journal of ophthalmology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 51 citations in OpenAlex.
- Integration of tear fluid biomarkers and machine learning for the early detection of keratoconus.BMC ophthalmology · 2025Article
- In-depth analysis of the tear fluid glycoproteome reveals diverse lacritin glycosylation and spliceoforms.The Journal of biological chemistry · 2025Article
- In-depth analysis of the tear fluid glycoproteome reveals diverse lacritin glycosylation and spliceoforms.bioRxiv : the preprint server for biology · 2025Article
- Galectin-3, a damage-associated molecular pattern, in tears of patients with vernal keratoconjunctivitis.Japanese journal of ophthalmology · 2023Article
- Galectins as Regulators of Corneal Inflammation.Current opinion in physiology · 2021Article
- Structural insight into the binding of human galectins to corneal keratan sulfate, its desulfated form and related saccharides.Scientific reports · 2020Article
- Disrupted Glycocalyx as a Source of Ocular Surface Biomarkers.Eye & contact lens · 2020Review
- Galectin-3 is an amplifier of the interleukin-1β-mediated inflammatory response in corneal keratinocytes.Immunology · 2018Article
- Galectin-3: One Molecule for an Alphabet of Diseases, from A to Z.International journal of molecular sciences · 2018Review
- Lectin-Glycan Interactions in Corneal Infection and Inflammation.Frontiers in immunology · 2018Review
- Galectin-3 enhances extracellular matrix associations and wound healing in monkey corneal epithelium.Experimental eye research · 2015Article
- Alteration of galectin-3 in tears of patients with dry eye disease.American journal of ophthalmology · 2015Observational
- The ocular surface epithelial barrier and other mechanisms of mucosal protection: from allergy to infectious diseases.Current opinion in allergy and clinical immunology · 2013Review
- Glycobiology of the ocular surface: mucins and lectins.Japanese journal of ophthalmology · 2013Review
- Adhesion/growth-regulatory galectins in the human eye: localization profiles and tissue reactivities as a standard to detect disease-associated alterations.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2012Article
- Image analysis tools for evaluation of microscopic views of immunohistochemically stained specimen in medical research-a review.Journal of medical systems · 2012Review
- Detection of differentially expressed wound-healing-related glycogenes in galectin-3-deficient mice.Investigative ophthalmology & visual science · 2009Article
- Glycoprotein 340 in normal human ocular surface tissues and tear film.Infection and immunity · 2006Article
- Galectin-3 as a regulator of the epithelial junction: Implications to wound repair and cancer.Tissue barriersReview
Corrections and comments
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Authors and funding
8 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND/
aimComponents of the tear fluid contribute to the biochemical defence system of the eye. To reveal whether the immune mediator and lipopolysaccharide binding galectin-3 is present in tears, tear samples were collected from eyes in healthy and pathological states. Investigation of expression of galectin-3 and galectin-3 reactive glycoligands in normal human conjunctival and corneal epithelia was also initiated as a step to understand the role of galectin-3 in ocular surface pathology.
methodsImmunoblot analysis using either a rabbit polyclonal or a mouse monoclonal antibody against galectin-3 was employed to detect galectin-3 in tear fluid. Galectin-3 expression in tissue specimens was detected by immunocytochemistry employing A1D6 mouse monoclonal antibody, and galectin-3 reactive glycoligands were visualised by lectin histochemistry using labelled galectin-3.
resultsGalectin-3 was found only in tears from patients with ocular surface disorders. It was expressed in normal corneal and conjunctival epithelia but not in lacrimal glands. Inflammatory leucocytes and goblet cells found in galectin-3 containing tear fluid also expressed galectin-3. Galectin-3 binding sites were detected on the surface of conjunctival and corneal epithelial cells co-localising with desmoglein.
conclusionsThis study revealed expression of galectin-3 in tear fluid obtained from patients with eye diseases. The role of this endogenous lectin (produced by inflammatory as well as epithelial cells) in antimicrobial action and inflammation modulation could be expected.
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