ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2001
Texture analysis of fluorescence lifetime images of AT- and GC-rich regions in nuclei.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
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- Hippocampal dendritic spines store-operated calcium entry and endoplasmic reticulum content is dynamic microtubule dependent.Scientific reports · 2025Article
- Contribution of advanced fluorescence nano microscopy towards revealing mitotic chromosome structure.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Review
- Purification, Preliminary Structure and Antitumor Activity of Exopolysaccharide Produced byMolecules (Basel, Switzerland) · 2018Article
- Live cell in vitro and in vivo imaging applications: accelerating drug discovery.Pharmaceutics · 2011Article
- Development of a membrane-bound random DNA sequence combinatorial array recognition surface (CARS).Journal of biomolecular techniques : JBT · 2010Article
- Spatiotemporal organization of AT- and GC-rich DNA and their association with transition proteins TP1 and TP2 in rat condensing spermatids.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2009Article
- Probing DNA hybridization in homogeneous solution and at interfaces via measurement of the intrinsic fluorescence decay time of a single label.Journal of fluorescence · 2008Article
- Studying inner ear protein-protein interactions using FRET and FLIM.Brain research · 2006Review
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Authors and funding
3 authors.
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Abstract
We used intensity and fluorescence lifetime microscopy (FLIM) of 3T3 nuclei to investigate the existence of AT-rich and GC-rich regions of the nuclear DNA. Hoechst 33258 (Ho) and 7-aminoactinomycin D (7-AAD) were used as fluorescence probes specific for AT and GC base pairs, respectively. YOYO-1 (Yo) was used as a dye that displays distinct fluorescence lifetimes when bound to AT or GC base pairs. We combined fluorescence imaging of Ho and 7-AAD with time-resolved measurements of Yo and took advantage of an additional information content of the time-resolved fluorescence. Because a single nucleus could not be stained and measured with all three dyes, we used texture analysis to compare the spatial distribution of AT-rich and GC-rich DNA in 100 nuclei in different phases of the cell cycle. The fluorescence intensity-based analysis of Ho- or 7-AAD-stained images indicates increased number and larger size of the DNA condensation centers in the G2/M-phases compared to G0/1-phases. The lifetime-based study of Yo-stained images suggests spatial separation of the AT- or GC-rich DNA regions in the G2/M-phase. Texture analysis of fluorescence intensity and lifetime images was used to quantitatively study the spatial change of condensation and separation of AT- and GC-rich DNA during the cell cycle.
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