ArticleProceedings of the National Academy of Sciences of the United States of America2001
Position within the host intron is critical for efficient processing of box C/D snoRNAs in mammalian cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
48 citing papers in PubMed, 100 citations in OpenAlex.
- Review
- Review
- SnoBIRD: a tool to identify C/D box snoRNAs and refine their annotation across all eukaryotes.Nucleic acids research · 2025Article
- RNA Through Time: From the Origin of Life to Therapeutic Frontiers in Transcriptomics and Epitranscriptional Medicine.International journal of molecular sciences · 2025Review
- NAP-seq reveals multiple classes of structured noncoding RNAs with regulatory functions.Nature communications · 2024Article
- Ribosome biogenesis factors-from names to functions.The EMBO journal · 2023Review
- Motif conservation, stability, and host gene expression are the main drivers of snoRNA expression across vertebrates.Genome research · 2023Article
- Maturation of small nucleolar RNAs: from production to function.RNA biology · 2023Review
- Emerging Data on the Diversity of Molecular Mechanisms Involving C/D snoRNAs.Non-coding RNA · 2021Review
- Developmental changes of rRNA ribose methylations in the mouse.RNA biology · 2020Article
- The cellular landscape of mid-size noncoding RNA.Wiley interdisciplinary reviews. RNA · 2019Review
- Polymorphisms in CEP68 gene associated with risk of immediate selective reactions to non-steroidal anti-inflammatory drugs.The pharmacogenomics journal · 2019Article
- Assembly and trafficking of box C/D and H/ACA snoRNPs.RNA biology · 2017Review
- C/D-box snoRNAs form methylating and non-methylating ribonucleoprotein complexes: Old dogs show new tricks.BioEssays : news and reviews in molecular, cellular and developmental biology · 2017Review
- Functions and mechanisms of long noncoding RNAs in lung cancer.OncoTargets and therapy · 2016Review
- DGCR8 Acts as an Adaptor for the Exosome Complex to Degrade Double-Stranded Structured RNAs.Molecular cell · 2015Article
- SNORD116 and SNORD115 change expression of multiple genes and modify each other's activity.Gene · 2015Article
- Identification of the specific interactors of the human lariat RNA debranching enzyme 1 protein.International journal of molecular sciences · 2015Article
- Human nonsense-mediated RNA decay initiates widely by endonucleolysis and targets snoRNA host genes.Genes & development · 2014Article
- Conserved sequence-specific lincRNA-steroid receptor interactions drive transcriptional repression and direct cell fate.Nature communications · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
In mammalian cells, all small nucleolar RNAs (snoRNAs) that guide rRNA modification are encoded within the introns of host genes. A database analysis of human box C/D snoRNAs revealed conservation of their intronic location, with a preference for 70-80 nt upstream of the 3' splice site. Transfection experiments showed that synthesis of gas5-encoded U75 and U76 snoRNAs dropped significantly for mutant constructs possessing longer or shorter spacers between the snoRNA and the 3' splice site. However, the position of the snoRNA did not affect splicing of the host intron. Substitution mutations within the spacer indicated that the length, but not the specific sequence, is important. A in vitro system that couples pre-mRNA splicing and processing of U75 has been developed. U75 synthesis in vitro depends on its box C and D sequences and requires an appropriate spacer length. Further mutational analyses both in vivo and in vitro, with subsequent mapping of the branch points, revealed that the critical distance is from the snoRNA coding region to the branch point, suggesting synergy between splicing and snoRNA release.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.