ArticleProceedings of the National Academy of Sciences of the United States of America2001
Identification of sites for exponential translation in living dendrites.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 122 citations in OpenAlex.
- Article
- A perspective on molecular signalling dysfunction, its clinical relevance and therapeutics in autism spectrum disorder.Experimental brain research · 2022Review
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- A Ribosomal Perspective on Neuronal Local Protein Synthesis.Frontiers in molecular neuroscience · 2022Review
- Comprehensive catalog of dendritically localized mRNA isoforms from sub-cellular sequencing of single mouse neurons.BMC biology · 2019Article
- Random Neuronal Networks show homeostatic regulation of global activity while showing persistent changes in specific connectivity paths to theta burst stimuli.Scientific reports · 2018Article
- Intracellular RNA-tracking methods.Open biology · 2018Review
- Reducing eIF4E-eIF4G interactions restores the balance between protein synthesis and actin dynamics in fragile X syndrome model mice.Science signaling · 2017Article
- Article
- Screening the Molecular Framework Underlying Local Dendritic mRNA Translation.Frontiers in molecular neuroscience · 2017Review
- Article
- Functionally diverse dendritic mRNAs rapidly associate with ribosomes following a novel experience.Nature communications · 2014Article
- A transgenic mouse line for collecting ribosome-bound mRNA using the tetracycline transactivator system.Frontiers in molecular neuroscience · 2014Article
- Dendritic glutamate receptor mRNAs show contingent local hotspot-dependent translational dynamics.Cell reports · 2013Article
- Fragile X mental retardation protein and synaptic plasticity.Molecular brain · 2013Review
- Quantitative biology of single neurons.Journal of the Royal Society, Interface · 2012Review
- Perspectives on cell reprogramming with RNA.Trends in biotechnology · 2012Review
- Cytoplasmic intron sequence-retaining transcripts can be dendritically targeted via ID element retrotransposons.Neuron · 2011Article
- mRNA for the EAAC1 subtype of glutamate transporter is present in neuronal dendrites in vitro and dramatically increases in vivo after a seizure.Neurochemistry international · 2011Article
- Fragile X related protein 1 clusters with ribosomes and messenger RNAs at a subset of dendritic spines in the mouse hippocampus.PloS one · 2011Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Neuronal processes contain mRNAs and membrane structures, and some forms of synaptic plasticity seem to require protein synthesis in dendrites of hippocampal neurons. To quantitate dendritic protein synthesis, we used multiphoton microscopy of green fluorescent protein synthesized in living isolated dendrites. Transfection of dendrites with mRNA encoding green fluorescent protein resulted in fluorescence that exponentially increased on stimulation with a glutamate receptor agonist; a reaction attenuated by the translation inhibitors anisomycin and emetine. Comparable experiments on whole neurons revealed that (RS)-3,5-dihydroxy-phenylglycine 0.5 H(2)O (DHPG)-stimulated fluorescence was linear in cell bodies relative to the exponential increase seen in dendrites. Detailed spatial analysis of the subdendritic distribution of fluorescence revealed "hotspots," sites of dendritic translation that were spatially stable. However, detailed temporal analysis of these hotspots revealed heterogeneous rates of translation. A double-label protocol counterstaining for ribosomes indicated that sites of "fastest" translation correlated with increased ribosome density, consistent with ribosome subunit assembly for initiation, the first step of translation. We propose that dendrites have specific sites specialized for fast translation.
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