ArticleProceedings of the National Academy of Sciences of the United States of America2001
Redundant control of rereplication in fission yeast.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 58 citations in OpenAlex.
- The Crk4-Cyc4 complex regulates GThe EMBO journal · 2024Article
- Anatomy and evolution of a DNA replication origin.Chromosoma · 2021Article
- Regulation ofMolecular cancer research : MCR · 2017Article
- Mechanisms and regulation of DNA replication initiation in eukaryotes.Critical reviews in biochemistry and molecular biology · 2017Review
- Regulating DNA replication in eukarya.Cold Spring Harbor perspectives in biology · 2013Review
- Genomic instability in cancer.Cold Spring Harbor perspectives in biology · 2013Review
- Sequential steps in DNA replication are inhibited to ensure reduction of ploidy in meiosis.Molecular biology of the cell · 2013Article
- Dynamic association of ORCA with prereplicative complex components regulates DNA replication initiation.Molecular and cellular biology · 2012Article
- CRL4-DDB1-VPRBP ubiquitin ligase mediates the stress triggered proteolysis of Mcm10.Nucleic acids research · 2012Article
- Defining the phosphodiesterase superfamily members in rat brain microvessels.ACS chemical neuroscience · 2011Article
- Cdt1 proteolysis is promoted by dual PIP degrons and is modulated by PCNA ubiquitylation.Nucleic acids research · 2011Article
- Prevention of DNA re-replication in eukaryotic cells.Journal of molecular cell biology · 2011Review
- Specific replication origins promote DNA amplification in fission yeast.Journal of cell science · 2010Article
- Preferential re-replication of Drosophila heterochromatin in the absence of geminin.PLoS genetics · 2010Article
- Geminin stabilizes Cdt1 during meiosis in Xenopus oocytes.The Journal of biological chemistry · 2009Article
- A mutation in the tomato DDB1 gene affects cell and chloroplast compartment size and CDT1 transcript.Plant signaling & behavior · 2008Article
- Checkpoint effects and telomere amplification during DNA re-replication in fission yeast.BMC molecular biology · 2007Article
- C. elegans CUL-4 prevents rereplication by promoting the nuclear export of CDC-6 via a CKI-1-dependent pathway.Current biology : CB · 2007Article
- The CENP-B homolog, Abp1, interacts with the initiation protein Cdc23 (MCM10) and is required for efficient DNA replication in fission yeast.Cell division · 2006Article
- DNA damage induces Cdt1 proteolysis in fission yeast through a pathway dependent on Cdt2 and Ddb1.EMBO reports · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The initiation of DNA replication at replication origins in eukaryotic cells is tightly controlled to ensure that the genome is duplicated only once each cell cycle. We present evidence that in fission yeast, independent regulation of two essential components of the initiation complex, Cdc18 and Cdt1, contributes to the prevention of reinitiation of DNA replication. Cdc18 is negatively controlled by cyclin-dependent kinase (CDK) phosphorylation, but low level expression of a mutant form of Cdc18 lacking CDK phosphorylation sites (Cdc18(CDK)) is not sufficient to induce rereplication. Similar to Cdc18, Cdt1 is expressed periodically in the cell cycle, accumulating in the nucleus in G(1) and declining in G(2). When Cdt1 is expressed constitutively from an ectopic promoter, it accumulates in the nucleus throughout the cell cycle but does not promote reinitiation. However, constitutive expression of Cdt1, together with Cdc18(CDK), is sufficient to induce extra rounds of DNA replication in the absence of mitosis. Significantly greater levels of rereplication can be induced by coexpression of Cdc18(CDK) and a Cdt1 mutant lacking a conserved C-terminal motif. In contrast, uncontrolled DNA replication does not occur when either mutant protein is expressed in the absence of the other. Constitutive expression of wild-type or mutant Cdt1 also leads to an increase in the levels of Cdc18(CDK), possibly as a result of increased protein stability. Our data are consistent with the hypothesis that control of rereplication depends on a redundant mechanism in which negative regulation of Cdt1 functions in parallel with the negative regulation of Cdc18.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.