ArticleGenetics2001
Recombination events in Neurospora crassa may cross a translocation breakpoint by a template-switching mechanism.
Article in Genetics, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 20 citations in OpenAlex.
- Repeated strand invasion and extensive branch migration are hallmarks of meiotic recombination.Molecular cell · 2021Article
- Meiotic Recombination in Neurospora crassa Proceeds by Two Pathways with Extensive Holliday Junction Migration.PloS one · 2016Article
- Contrasted patterns of crossover and non-crossover at Arabidopsis thaliana meiotic recombination hotspots.PLoS genetics · 2013Article
- Residual recombination in Neurospora crassa spo11 deletion homozygotes occurs during meiosis.Molecular genetics and genomics : MGG · 2013Article
- Use of fluorescent protein to analyse recombination at three loci in Neurospora crassa.Fungal genetics and biology : FG & B · 2012Article
- A crossover hotspot near his-3 in Neurospora crassa is a preferential recombination termination site.Molecular genetics and genomics : MGG · 2012Article
- Genome-wide analysis of heteroduplex DNA in mismatch repair-deficient yeast cells reveals novel properties of meiotic recombination pathways.PLoS genetics · 2011Article
- Chromosome pairing and meiotic recombination in Neurospora crassa spo11 mutants.Current genetics · 2006Article
- Recombination at his-3 in Neurospora declines exponentially with distance from the initiator, cog.Genetics · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To assist investigation of the effect of sequence heterology on recombination in Neurospora crassa, we inserted the Herpes simplex thymidine kinase gene (TK) as an unselected marker on linkage group I, giving a gene order of Cen-his-3-TK-cog-lpl. We show here that in crosses heterozygous for TK, conversion of a his-3 allele on one homolog is accompanied by transfer of the heterologous sequence between cog and his-3 from the other homolog, indicating that recombination is initiated centromere-distal of TK. We have identified a 10-nucleotide motif in the cog region that, although unlikely to be sufficient for hotspot activity, is required for high-frequency recombination and, because conversion of silent sequence markers declines on either side, may be the recombination initiation site. Additionally, we have mapped conversion tracts in His(+) progeny of a translocation heterozygote, in which the translocation breakpoint separates cog from the 5' end of his-3. We present molecular evidence of recombination on both sides of the breakpoint. Because recombination is initiated close to cog and the event must therefore cross the translocation breakpoint, we suggest that template switching occurs in some recombination events, with repair synthesis alternating between use of the homolog and the initiating chromatid as template.
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