ArticleBiophysical journal2001
Motility of single one-headed kinesin molecules along microtubules.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Biased movement of monomeric kinesin-3 KLP-6 explained by a symmetric Brownian ratchet model.Biophysical journal · 2025Article
- Structure-based molecular simulations reveal the enhancement of biased Brownian motions in single-headed kinesin.PLoS computational biology · 2013Article
- A single protofilament is sufficient to support unidirectional walking of dynein and kinesin.PloS one · 2012Article
- Mechanism of processive movement of monomeric and dimeric kinesin molecules.International journal of biological sciences · 2010Review
- Single molecular observation of self-regulated kinesin motility.Biochemistry · 2010Article
- One-dimensional Brownian motion of charged nanoparticles along microtubules: a model system for weak binding interactions.Biophysical journal · 2010Article
- Diffusive movement of processive kinesin-1 on microtubules.Traffic (Copenhagen, Denmark) · 2009Article
- The diffusive interaction of microtubule binding proteins.Current opinion in cell biology · 2009Review
- Diffusion and directed movement: in vitro motile properties of fission yeast kinesin-14 Pkl1.The Journal of biological chemistry · 2008Article
- Two distinct modes of processive kinesin movement in mixtures of ATP and AMP-PNP.The Journal of general physiology · 2007Article
- Identification of a strong binding site for kinesin on the microtubule using mutant analysis of tubulin.The EMBO journal · 2006Article
- Full-length dimeric MCAK is a more efficient microtubule depolymerase than minimal domain monomeric MCAK.Molecular biology of the cell · 2006Article
- Processive movement of single kinesins on crowded microtubules visualized using quantum dots.The EMBO journal · 2006Article
- The E-hook of tubulin interacts with kinesin's head to increase processivity and speed.Biophysical journal · 2005Article
- Biased binding of single molecules and continuous movement of multiple molecules of truncated single-headed kinesin.Biophysical journal · 2005Article
- A one-headed class V myosin molecule develops multiple large (approximately 32-nm) steps successively.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- The human chromokinesin Kid is a plus end-directed microtubule-based motor.The EMBO journal · 2003Article
- Coordination of kinesin's two heads studied with mutant heterodimers.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The motility of single one-headed kinesin molecules (K351 and K340), which were truncated fragments of Drosophila two-headed kinesin, has been tested using total internal reflection fluorescence microscopy. One-headed kinesin fragments moved continuously along the microtubules. The maximum distance traveled until the fragments dissociated from the microtubules for both K351 and K340 was approximately 600 nm. This value is considerably larger than the space resolution of the measurement system (SD approximately 30 nm). Although the movements of the fragments fluctuated in forward and backward directions, statistical analysis showed that the average movements for both K340 and K351 were toward the plus end of the microtubules, i.e., forward direction. When BDTC (a 1.3-S subunit of Propionibacterium shermanii transcarboxylase, which binds weakly to a microtubule), was fused to the tail (C-terminus) of K351, its movement was enhanced, smooth, and unidirectional, similar to that of the two-headed kinesin fragment, K411. However, the travel distance and velocity of K351BDTC molecules were approximately 3-fold smaller than that of K411. These observations suggest that a single kinesin head has basal motility, but coordination between the two heads is necessary for stabilizing the basal motility for the normal level of kinesin processivity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.