ArticleBiophysical journal2001
Purification of native myosin filaments from muscle.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Myosin Filaments of Vertebrate Skeletal and Cardiac Muscle are Highly Similar, but not Identical.bioRxiv : the preprint server for biology · 2026Article
- A simple and rapid preparation of smooth muscle myosin 2 for the electron microscopic analysis.Applied microscopy · 2024Article
- Article
- Approaches to altering particle distributions in cryo-electron microscopy sample preparation.Acta crystallographica. Section D, Structural biology · 2018Review
- Lessons from a tarantula: new insights into muscle thick filament and myosin interacting-heads motif structure and function.Biophysical reviews · 2017Review
- Structure of myosin filaments from relaxedScience advances · 2016Article
- A method for 3D-reconstruction of a muscle thick filament using the tilt series images of a single filament electron tomogram.Journal of structural biology · 2014Article
- Isolation, electron microscopy and 3D reconstruction of invertebrate muscle myofilaments.Methods (San Diego, Calif.) · 2012Review
- Head-head interaction characterizes the relaxed state of Limulus muscle myosin filaments.Journal of molecular biology · 2009Article
- Ca2+ -induced tropomyosin movement in scallop striated muscle thin filaments.Journal of molecular biology · 2008Article
- Blebbistatin stabilizes the helical order of myosin filaments by promoting the switch 2 closed state.Biophysical journal · 2008Article
- Head-head and head-tail interaction: a general mechanism for switching off myosin II activity in cells.Molecular biology of the cell · 2008Article
- Three-dimensional structure of vertebrate cardiac muscle myosin filaments.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Characterization of the myosin-based source for second-harmonic generation from muscle sarcomeres.Biophysical journal · 2006Article
- Synthetic thick filaments: A new avenue for better understanding the myosin super-relaxed state in healthy, diseased, and mavacamten-treated cardiac systems.The Journal of biological chemistryArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Analysis of the structure and function of native thick (myosin-containing) filaments of muscle has been hampered in the past by the difficulty of obtaining a pure preparation. We have developed a simple method for purifying native myosin filaments from muscle filament suspensions. The method involves severing thin (actin-containing) filaments into short segments using a Ca(2+)-insensitive fragment of gelsolin, followed by differential centrifugation to purify the thick filaments. By gel electrophoresis, the purified thick filaments show myosin heavy and light chains together with nonmyosin thick filament components. Contamination with actin is below 3.5%. Electron microscopy demonstrates intact thick filaments, with helical cross-bridge order preserved, and essentially complete removal of thin filaments. The method has been developed for striated muscles but can also be used in a modified form to remove contaminating thin filaments from native smooth muscle myofibrils. Such preparations should be useful for thick filament structural and biochemical studies.
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Registered trials
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