ArticleJournal of the American Society for Mass Spectrometry2001
The influence of electrostatic interactions on the detection of heme-globin complexes in ESI-MS.
Article in Journal of the American Society for Mass Spectrometry, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Nanoflow Size Exclusion Chromatography-Native Mass Spectrometry of Intact Proteoforms and Protein Complexes.Analytical chemistry · 2025Article
- Stabilization of Protein Interactions through Electrospray Additives in Negative Ion Mode Native Mass Spectrometry.Analytical chemistry · 2025Article
- Mass Spectrometric Analysis of Antibody-Epitope Peptide Complex Dissociation: Theoretical Concept and Practical Procedure of Binding Strength Characterization.Molecules (Basel, Switzerland) · 2020Article
- Comparative analysis of oxy-hemoglobin and aquomet-hemoglobin by hydrogen/deuterium exchange mass spectrometry.Journal of the American Society for Mass Spectrometry · 2013Article
- Super-atmospheric pressure electrospray ion source: applied to aqueous solution.Journal of the American Society for Mass Spectrometry · 2011Article
- Heme binding in gas-phase holo-myoglobin cations: distal becomes proximal?Journal of the American Society for Mass Spectrometry · 2011Article
- Direct ionization of large proteins and protein complexes by desorption electrospray ionization-mass spectrometry.Analytical chemistry · 2011Article
- Characterization of acid-induced protein conformational changes and noncovalent complexes in solution by using coldspray ionization mass spectrometry.Journal of the American Society for Mass Spectrometry · 2009Article
- Selective detection of specific protein-ligand complexes by electrosonic spray-precursor ion scan tandem mass spectrometry.Journal of the American Society for Mass Spectrometry · 2009Article
- Analysis of protein mixtures by electrospray mass spectrometry: effects of conformation and desolvation behavior on the signal intensities of hemoglobin subunits.Journal of the American Society for Mass Spectrometry · 2007Article
- Electrospray ionization mass spectrometry studies of noncovalent myosin VI complexes reveal a new specific calmodulin binding site.Journal of the American Society for Mass Spectrometry · 2005Article
- Collisional cooling enhances the ability to observe non-covalent interactions within the inducible nitric oxide synthase oxygenase domain: dimerization, complexation, and dissociation.Journal of the American Society for Mass Spectrometry · 2004Article
- Diffusion measurements by electrospray mass spectrometry for studying solution-phase noncovalent interactions.Journal of the American Society for Mass Spectrometry · 2003Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The heme-globin complexes of hemoglobin and myoglobin are investigated in positive-ion mode and negative-ion mode using a nano-ESI source coupled to a quadrupole ion trap MS and an orthogonal time-of-flight MS. The extent of dissociation of these noncovalent complexes upon collisional activation and thus their gas-phase stability is strongly dependent on the polarity of the ESI-MS experiment as well as on the charge of the prosthetic group (ferri-heme [Fe3+-heme]+ vs. ferro-heme [Fe2+-heme]+/-0). The results clearly point to the important role of electrostatic interactions on the gas phase stability of noncovalent complexes and therefore the ion signals observed in ESI-MS experiments.
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