ArticleMolecular and cellular biology2001
A truncation mutant of the 95-kilodalton subunit of transcription factor IIIC reveals asymmetry in Ty3 integration.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Structural basis of Ty3 retrotransposon integration at RNA Polymerase III-transcribed genes.Nature communications · 2021Article
- Light and shadow on the mechanisms of integration site selection in yeast Ty retrotransposon families.Current genetics · 2021Review
- Local features determine Ty3 targeting frequency at RNA polymerase III transcription start sites.Genome research · 2019Article
- Convergent evolution of integration site selection upstream of tRNA genes by yeast and amoeba retrotransposons.Nucleic acids research · 2018Article
- Retrotransposon targeting to RNA polymerase III-transcribed genes.Mobile DNA · 2018Review
- Integration site selection by retroviruses and transposable elements in eukaryotes.Nature reviews. Genetics · 2017Review
- Review
- RNA Polymerase III Advances: Structural and tRNA Functional Views.Trends in biochemical sciences · 2016Review
- Ty1 Integrase Interacts with RNA Polymerase III-specific Subcomplexes to Promote Insertion of Ty1 Elements Upstream of Polymerase (Pol) III-transcribed Genes.The Journal of biological chemistry · 2016Article
- A long terminal repeat retrotransposon of Schizosaccharomyces japonicus integrates upstream of RNA pol III transcribed genes.Mobile DNA · 2015Article
- RNA polymerase III-specific general transcription factor IIIC contains a heterodimer resembling TFIIF Rap30/Rap74.Nucleic acids research · 2013Article
- Retrotransposon profiling of RNA polymerase III initiation sites.Genome research · 2012Article
- Targeted gene insertion for molecular medicine.Journal of molecular medicine (Berlin, Germany) · 2008Review
- Retroviruses and yeast retrotransposons use overlapping sets of host genes.Genome research · 2005Article
- Genome-wide identification of Isw2 chromatin-remodeling targets by localization of a catalytically inactive mutant.Genes & development · 2005Article
- Article
- Local definition of Ty1 target preference by long terminal repeats and clustered tRNA genes.Genome research · 2004Article
- Integration by design.Proceedings of the National Academy of Sciences of the United States of America · 2003Review
- Controlling integration specificity of a yeast retrotransposon.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Position-specific integration of the retroviruslike element Ty3 near the transcription initiation sites of tRNA genes requires transcription factors IIIB and IIIC (TFIIIB and TFIIIC). Using a genetic screen, we isolated a mutant with a truncated 95-kDa subunit of TFIIIC (TFIIIC95) that reduced the apparent retrotransposition of Ty3 into a plasmid-borne target site between two divergently transcribed tRNA genes. Although TFIIIC95 is conserved and essential, no defect in growth or transcription of tRNAs was detected in the mutant. Steps of the Ty3 life cycle, such as protein expression, proteolytic processing, viruslike particle formation, and reverse transcription, were not affected by the mutation. However, Ty3 integration into a divergent tDNA target occurred exclusively in one orientation in the mutant strain. Investigation of this orientation bias showed that TFIIIC95 and Ty3 integrase interacted in two-hybrid and glutathione S-transferase pulldown assays and that interaction with the mutant TFIIIC95 protein was attenuated. The orientation bias observed here suggests that even for wild-type Ty3, the protein complexes associated with the long terminal repeats are not equivalent in vivo.
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Registered trials
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